The Serratia grimesii outer membrane vesicles‐associated grimelysin triggers bacterial invasion of eukaryotic cells
Sign Up to like & getrecommendations! Published in 2020 at "Cell Biology International"
DOI: 10.1002/cbin.11435
Abstract: Serratia grimesii are facultative pathogenic bacteria that can penetrate a wide range of host cells and cause infection, especially in immunocompromised patients. Previously, we have found that bacterial metalloprotease grimelysin is a potential virulence determinant… read more here.
Keywords: grimesii; serratia grimesii; outer membrane; grimelysin ... See more keywords
LITESEC-T3SS - Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution
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DOI: 10.1038/s41467-020-16169-w
Abstract: Many bacteria employ a type III secretion system (T3SS) injectisome to translocate proteins into eukaryotic host cells. Although the T3SS can efficiently export heterologous cargo proteins, a lack of target cell specificity currently limits its… read more here.
Keywords: t3ss; litesec t3ss; spatial temporal; eukaryotic cells ... See more keywords
Salmonella-based platform for efficient delivery of functional binding proteins to the cytosol
Sign Up to like & getrecommendations! Published in 2020 at "Communications Biology"
DOI: 10.1038/s42003-020-1072-4
Abstract: Protein-based affinity reagents (like antibodies or alternative binding scaffolds) offer wide-ranging applications for basic research and therapeutic approaches. However, whereas small chemical molecules efficiently reach intracellular targets, the delivery of macromolecules into the cytosol of… read more here.
Keywords: salmonella; secretion system; system; delivery ... See more keywords
Probing and manipulating intracellular membrane traffic by microinjection of artificial vesicles
Sign Up to like & getrecommendations! Published in 2017 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.1713524114
Abstract: Significance In eukaryotic cells, membranous organelles exchange trafficking vesicles that bud from the precursor and are transported to the target membrane where they fuse. Many of the required proteins are known. Moreover, some steps such… read more here.
Keywords: membrane traffic; eukaryotic cells; probing manipulating; artificial vesicles ... See more keywords
Moving toward molecular mechanisms for chemotaxis in eukaryotic cells
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Biology of the Cell"
DOI: 10.1091/mbc.e19-07-0393
Abstract: It is a tremendous honor to receive the 2019 E.B. Wilson Award and be recognized for my work on chemotaxis in eukaryotic cells. In writing this essay, I hope to achieve three aims: 1) to… read more here.
Keywords: molecular mechanisms; mechanisms chemotaxis; chemotaxis eukaryotic; toward molecular ... See more keywords
Oligosaccharyltransferase structures provide novel insight into the mechanism of asparagine-linked glycosylation in prokaryotic and eukaryotic cells.
Sign Up to like & getrecommendations! Published in 2019 at "Glycobiology"
DOI: 10.1093/glycob/cwy093
Abstract: Asparagine-linked (N-linked) glycosylation is one of the most common protein modification reactions in eukaryotic cells, occurring upon the majority of proteins that enter the secretory pathway. X-ray crystal structures of the single subunit OSTs from… read more here.
Keywords: mechanism; microscopy; linked glycosylation; glycosylation ... See more keywords
Building peptidoglycan inside eukaryotic cells: A view from symbiotic and pathogenic bacteria.
Sign Up to like & getrecommendations! Published in 2020 at "Molecular Microbiology"
DOI: 10.1111/mmi.14452
Abstract: The peptidoglycan (PG), as the exoskeleton of most prokaryotes, maintains a defined shape and ensures cell integrity against the high internal turgor pressure. These important roles have attracted researchers to target PG metabolism in order… read more here.
Keywords: pathogenic bacteria; building peptidoglycan; inside eukaryotic; eukaryotic cells ... See more keywords
Signal and regulatory effects of methylglyoxal in eukaryotic cells (review)
Sign Up to like & getrecommendations! Published in 2017 at "Applied Biochemistry and Microbiology"
DOI: 10.1134/s0003683817030103
Abstract: Methylglyoxal (MG) is one of the physiological glucose metabolites formed in living organisms. The data on the influence of MG on different internal systems of eukaryotic cells, including the central signaling pathways, are been discussed… read more here.
Keywords: regulatory effects; signal regulatory; effects methylglyoxal; methylglyoxal eukaryotic ... See more keywords
The Opposite Effects of ROCK and Src Kinase Inhibitors on Susceptibility of Eukaryotic Cells to Invasion by Bacteria Serratia grimesii
Sign Up to like & getrecommendations! Published in 2019 at "Biochemistry (Moscow)"
DOI: 10.1134/s0006297919060099
Abstract: Bacterial internalization into eukaryotic cells is ensured by a sophisticated interplay of bacterial and host cell factors. Being a part of cell environment, opportunistic intracellular bacteria have developed various mechanisms providing their interaction with cell… read more here.
Keywords: src kinase; invasion; eukaryotic cells; rock ... See more keywords
Early “reduction to practice” of the CRISPR–Cas9 invention in eukaryotic cells
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Genetics"
DOI: 10.3389/fgene.2022.1009688
Abstract: The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)–Cas (CRISPR-associated proteins) are microbial adaptive immune mechanisms that have revolutionized many areas of Life Sciences research and innovation and potentially could transform the lives of patients with… read more here.
Keywords: crispr cas9; dna; crispr; cas9 ... See more keywords
Bacterial Actin-Specific Endoproteases Grimelysin and Protealysin as Virulence Factors Contributing to the Invasive Activities of Serratia
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DOI: 10.3390/ijms21114025
Abstract: The article reviews the discovery, properties and functional activities of new bacterial enzymes, proteases grimelysin (ECP 32) of Serratia grimesii and protealysin of Serratia proteamaculans, characterized by both a highly specific “actinase” activity and their… read more here.
Keywords: actin; invasion; protealysin; grimelysin protealysin ... See more keywords