SMOOT libraries and phage-induced directed evolution of Cas9 to engineer reduced off-target activity
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DOI: 10.1371/journal.pone.0231716
Abstract: RNA-guided endonucleases such as Cas9 provide efficient on-target genome editing in cells but may also cleave at off-target loci throughout the genome. Engineered variants of Streptococcus pyogenes Cas9 (SpCas9) have been developed to globally reduce… read more here.
Keywords: activity; target; directed evolution; evolution cas9 ... See more keywords