Directed Evolution of Proteins Based on Mutational Scanning.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7366-8_6
Abstract: Directed evolution has emerged as one of the most effective protein engineering methods in basic research as well as in applications in synthetic organic chemistry and biotechnology. The successful engineering of protein activity, allostery, binding… read more here.
Keywords: evolution proteins; mutational scanning; chemistry; directed evolution ... See more keywords
Automated continuous evolution of proteins in vivo.
Sign Up to like & getrecommendations! Published in 2020 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.0c00135
Abstract: We present automated continuous evolution (ACE), a platform for the hands-free directed evolution of biomolecules. ACE pairs OrthoRep, a genetic system for continuous targeted mutagenesis of user-selected genes in vivo, with eVOLVER, a scalable and… read more here.
Keywords: evolution; evolution proteins; automated continuous; continuous evolution ... See more keywords