Combined evolutionary engineering and genetic manipulation improve low pH tolerance and butanol production in a synthetic microbial Clostridium community
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27333
Abstract: Synthetic microbial communities have become a focus of biotechnological research since they can overcome several of the limitations of single‐specie cultures. A paradigmatic example is Clostridium cellulovorans DSM 743B, which can decompose lignocellulose but cannot… read more here.
Keywords: evolutionary engineering; butanol; synthetic microbial; clostridium ... See more keywords
Genomic, transcriptomic and physiological analyses of silver‐resistant Saccharomyces cerevisiae obtained by evolutionary engineering
Sign Up to like & getrecommendations! Published in 2020 at "Yeast"
DOI: 10.1002/yea.3514
Abstract: Silver is a non‐essential metal used in medical applications as an antimicrobial agent, but it is also toxic for biological systems. To investigate the molecular basis of silver resistance in yeast, we employed evolutionary engineering… read more here.
Keywords: genomic transcriptomic; evolutionary engineering; saccharomyces cerevisiae; analysis results ... See more keywords
Evolutionary Engineering of a Cp*Rh(III) Complex-Linked Artificial Metalloenzyme with a Chimeric β-Barrel Protein Scaffold
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.3c00581
Abstract: Evolutionary engineering of our previously reported Cp*Rh(III)-linked artificial metalloenzyme was performed based on a DNA recombination strategy to improve its catalytic activity toward C(sp2)–H bond functionalization. Improved scaffold design was achieved with α-helical cap domains… read more here.
Keywords: evolutionary engineering; artificial metalloenzyme; metalloenzyme; iii ... See more keywords
In Vitro Gut Modeling as a Tool for Adaptive Evolutionary Engineering of Lactiplantibacillus plantarum
Sign Up to like & getrecommendations! Published in 2021 at "mSystems"
DOI: 10.1128/msystems.01085-20
Abstract: Improvement of bacterial strains in regard to specific abiotic environmental factors is broadly used to enhance strain characteristics for processing and product quality. However, there is currently no multidimensional probiotic strain improvement approach for both… read more here.
Keywords: microbiota; strain; improvement; evolutionary engineering ... See more keywords
Evolutionary Engineering in Chemostat Cultures for Improved Maltotriose Fermentation Kinetics in Saccharomyces pastorianus Lager Brewing Yeast
Sign Up to like & getrecommendations! Published in 2017 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2017.01690
Abstract: The lager brewing yeast Saccharomyces pastorianus, an interspecies hybrid of S. eubayanus and S. cerevisiae, ferments maltotriose, maltose, sucrose, glucose and fructose in wort to ethanol and carbon dioxide. Complete and timely conversion (“attenuation”) of… read more here.
Keywords: evolutionary engineering; lager brewing; engineering; maltotriose fermentation ... See more keywords
Physiological and Molecular Characterization of an Oxidative Stress-Resistant Saccharomyces cerevisiae Strain Obtained by Evolutionary Engineering
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.822864
Abstract: Oxidative stress is a major stress type observed in yeast bioprocesses, resulting in a decrease in yeast growth, viability, and productivity. Thus, robust yeast strains with increased resistance to oxidative stress are in highly demand… read more here.
Keywords: evolutionary engineering; stress; oxidative stress; strain ... See more keywords
Selecting the Best: Evolutionary Engineering of Chemical Production in Microbes
Sign Up to like & getrecommendations! Published in 2018 at "Genes"
DOI: 10.3390/genes9050249
Abstract: Microbial cell factories have proven to be an economical means of production for many bulk, specialty, and fine chemical products. However, we still lack both a holistic understanding of organism physiology and the ability to… read more here.
Keywords: engineering; evolutionary engineering; production; selecting best ... See more keywords
Novel Evolutionary Engineering Approach to Alter Substrate Specificity of Disaccharide Transporter Mal11 in Saccharomyces cerevisiae
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Fungi"
DOI: 10.3390/jof8040358
Abstract: A major challenge in the research of transport proteins is to understand how single amino acid residues contribute to their structure and biological function. Amino acid substitutions that result in a selective advantage in adaptive… read more here.
Keywords: evolutionary engineering; alter substrate; disaccharide transporter; mal11 ... See more keywords