Impact of the isoelectric point of model parvoviruses on viral retention in anion‐exchange chromatography
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27555
Abstract: Anion‐exchange chromatography (AEX) is used in the downstream purification of monoclonal antibodies to remove impurities and potential viral contamination based on electrostatic interactions. Although the isoelectric point (pI) of viruses is considered a key factor… read more here.
Keywords: isoelectric point; anion exchange; exchange chromatography;
Modeling the impact of amino acid substitution in a monoclonal antibody on cation exchange chromatography
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27798
Abstract: A vital part of biopharmaceutical research is decision making around which lead candidate should be progressed in early‐phase development. When multiple antibody candidates show similar biological activity, developability aspects are taken into account to ease… read more here.
Keywords: cation exchange; antibody; amino acid; exchange chromatography ... See more keywords
Enrichment of adeno-associated virus serotype 5 full capsids by anion exchange chromatography with dual salt elution gradients.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.28453
Abstract: Adeno-associated virus-based gene therapies have demonstrated substantial therapeutic benefit for the treatment of genetic disorders. In manufacturing processes, viral capsids are produced with and without the encapsidated gene of interest. Capsids devoid of the gene… read more here.
Keywords: associated virus; adeno associated; full capsids; anion exchange ... See more keywords
A fast, single column high performance anion exchange chromatography with pulsed amperometric detection method for determination of saccharides in atmospheric aerosol samples.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of separation science"
DOI: 10.1002/jssc.202100842
Abstract: Saccharides, especially anhydro sugars present in atmospheric aerosols, can be used as tracers to track sources of atmospheric aerosol. High-performance anion-exchange chromatography with pulsed amperometric detection is a commonly used technique for determining these saccharides,… read more here.
Keywords: exchange chromatography; performance anion; anion exchange; high performance ... See more keywords
Mechanistic modeling of cation exchange chromatography scale-up considering packing inhomogeneities.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of separation science"
DOI: 10.1002/jssc.202300031
Abstract: In process development and characterization, the scale-up of chromatographic steps is a crucial part and brings a number of challenges. Usually, scale-down models are used to represent the process step, and constant column properties are… read more here.
Keywords: scale; exchange chromatography; mechanistic modeling; cation exchange ... See more keywords
Quantitative Kinetic Characterization of Glycoside Hydrolases Using High-Performance Anion-Exchange Chromatography (HPAEC).
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6899-2_2
Abstract: High-performance anion-exchange chromatography coupled to pulsed amperometric detection (HPAEC-PAD) is a powerful analytical technique enabling the high-resolution separation and sensitive quantification of oligosaccharides. Here, we describe a general method for the determination of glycoside hydrolase… read more here.
Keywords: hpaec; anion exchange; performance anion; exchange chromatography ... See more keywords
Purification of Bacteriophages Using Anion-Exchange Chromatography.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7343-9_5
Abstract: In bacteriophage research and therapy, most applications ask for highly purified phage suspensions. The standard technique for this is ultracentrifugation using cesium chloride gradients. This technique is cumbersome, elaborate and expensive. Moreover, it is unsuitable… read more here.
Keywords: purification bacteriophages; anion exchange; exchange chromatography;
Acquisition of enzymatic progress curves in real time by quenching-free ion exchange chromatography.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2021.114523
Abstract: We describe a quenching-free, 'online' ion exchange chromatography (oIEC) method for the quantitative analysis of enzymatic reactions in real-time. We show that separate quenching of the ongoing reaction performed conventionally is not required, since enzymatic… read more here.
Keywords: exchange chromatography; ion exchange; real time; quenching free ... See more keywords
Biomedical copper speciation in relation to Wilson's disease using strong anion exchange chromatography coupled to triple quadrupole inductively coupled plasma mass spectrometry.
Sign Up to like & getrecommendations! Published in 2020 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2019.11.033
Abstract: Biomedical analytical methods often rely on indirect measurements, such as immunoassays, which can lack effective metrological traceability. In the nephelometric determination of ceruloplasmin (Cp), an important protein whose circulating level is altered in Wilson's disease… read more here.
Keywords: coupled triple; anion exchange; strong anion; copper ... See more keywords
Analysis of added phosphates in hake fillets by ion-exchange chromatography: A case study of false positives induced by nucleotides coelution.
Sign Up to like & getrecommendations! Published in 2021 at "Food chemistry"
DOI: 10.1016/j.foodchem.2021.130841
Abstract: This study aimed to optimize an ion-exchange chromatography method for an accurate quantification of phosphates. Coelution of nucleotides was detected following the quantification of small amounts of di- and trimetaphosphates in hake fillets. In some… read more here.
Keywords: hake fillets; exchange chromatography; study; ion exchange ... See more keywords
Enhancing recovery of recombinant hepatitis B surface antigen in lab-scale and large-scale anion-exchange chromatography by optimizing the conductivity of buffers.
Sign Up to like & getrecommendations! Published in 2018 at "Protein expression and purification"
DOI: 10.1016/j.pep.2017.08.011
Abstract: In biopharmaceutical science, ion-exchange chromatography (IEC) is a well-known purification technique to separate the impurities such as host cell proteins from recombinant proteins. However, IEC is one of the limiting steps in the purification process… read more here.
Keywords: recovery; recombinant hepatitis; conductivity; exchange ... See more keywords