Direct coupling of size exclusion chromatography and mass spectrometry for the characterization of complex monoclonal antibody products
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Separation Science"
DOI: 10.1002/jssc.202200075
Abstract: The present study describes the possibilities offered by an innovative bioinert size exclusion chromatography column for size variant characterization of complex monoclonal antibody products. This size exclusion chromatography column includes a novel column hardware surface.… read more here.
Keywords: monoclonal antibody; size; size exclusion; exclusion chromatography ... See more keywords
Microfluidic Size Exclusion Chromatography (μSEC) for Extracellular Vesicles and Plasma Protein Separation.
Sign Up to like & getrecommendations! Published in 2022 at "Small"
DOI: 10.1002/smll.202104470
Abstract: Extracellular vesicles (EVs) are recognized as next generation diagnostic biomarkers due to their disease-specific biomolecular cargoes and importance in cell-cell communications. A major bottleneck in EV sample preparation is the inefficient and laborious isolation of… read more here.
Keywords: extracellular vesicles; chromatography sec; sec; size exclusion ... See more keywords
High-Throughput Nano-Scale Characterization of Membrane Proteins Using Fluorescence-Detection Size-Exclusion Chromatography.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9624-7_17
Abstract: Structural biology has revealed predicting heterologous expression levels, homogeneity, and stability of a protein from its primary structure are exceedingly difficult. Membrane proteins, in particular, present numerous challenges that make obtaining milligram quantities of quality… read more here.
Keywords: membrane proteins; characterization membrane; exclusion chromatography; biology ... See more keywords
Advances on Size Exclusion Chromatography and Applications on the Analysis of Protein Biopharmaceuticals and Protein Aggregates: A Mini Review
Sign Up to like & getrecommendations! Published in 2017 at "Chromatographia"
DOI: 10.1007/s10337-017-3380-5
Abstract: Biopharmaceutical drugs are large, complex protein molecules derived from living cells. The characterization of the protein-based products is a complicated and challenging task necessary to guarantee their efficacy and safety. A major concern in manufacturing… read more here.
Keywords: protein; protein aggregates; protein biopharmaceuticals; exclusion chromatography ... See more keywords
Quantification and varietal variation of low molecular weight glutenin subunits (LMW-GS) using size exclusion chromatography
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Food Science and Technology"
DOI: 10.1007/s13197-017-2995-8
Abstract: Crude glutenin of four commercial wheat varieties viz. C 306, HI 977, HW 2004 and PBW 550 of diverse origin and breadmaking quality were fractionated by size-exclusion chromatography into three fractions of decreasing molecular weights.… read more here.
Keywords: exclusion chromatography; size exclusion; quantification varietal;
Monitoring WWTP performance using size-exclusion chromatography with simultaneous UV and fluorescence detection to track recalcitrant wastewater fractions.
Sign Up to like & getrecommendations! Published in 2019 at "Chemosphere"
DOI: 10.1016/j.chemosphere.2018.09.099
Abstract: A trial monitoring of a typical full-scale municipal WWTP in Central Finland was aimed to explore applicability of high performance liquid chromatography - size exclusion chromatography (HPSEC) with simultaneous UV and fluorescence detection as a… read more here.
Keywords: fluorescence; exclusion chromatography; simultaneous fluorescence; wastewater ... See more keywords
Investigation of cellulose nitrate and cellulose acetate plastics in museum collections using ion chromatography and size exclusion chromatography
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Cultural Heritage"
DOI: 10.1016/j.culher.2018.05.011
Abstract: Abstract One of the goals of the Preservation of Plastics Project at the Getty Conservation Institute is to investigate preventative and conservation strategies for objects made of cellulose ester plastics. To achieve this goal, several… read more here.
Keywords: chromatography; ion chromatography; cellulose nitrate; size exclusion ... See more keywords
Pectin characterisation using size exclusion chromatography: A comparison of ELS and RI detection.
Sign Up to like & getrecommendations! Published in 2018 at "Food chemistry"
DOI: 10.1016/j.foodchem.2018.01.087
Abstract: A high-performance size-exclusion chromatography (HPSEC) method coupled to Evaporative Light Scattering (ELS) and Refractive Index (RI) detectors were evaluated and compared for the molecular mass (Mw) estimation of pectin in a wide range (0.342-805 kDa). Instrumental… read more here.
Keywords: exclusion chromatography; pectin characterisation; size exclusion;
Optimized asphaltene separation by online coupling of size exclusion chromatography and ultrahigh resolution mass spectrometry
Sign Up to like & getrecommendations! Published in 2018 at "Fuel"
DOI: 10.1016/j.fuel.2017.11.054
Abstract: Abstract The asphaltene fraction is the heaviest part of a crude oil and is obtained as the fraction which contains compounds that are not soluble in paraffinic solvents such as n -heptane. Due to the… read more here.
Keywords: exclusion chromatography; separation; size exclusion;
Development of a method to analyze the complexes of enoxaparin and platelet factor 4 with size‐exclusion chromatography
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Pharmaceutical and Biomedical Analysis"
DOI: 10.1016/j.jpba.2018.11.018
Abstract: HighlightsA method was developed to analyze the complex patterns of enoxaparin/PF4.The analytical method was developed based on size‐exclusion chromatography.Stability, incubation time and ratios of enoxaparin/FP4 was investigated.This method monitors the capacity of enoxaparin forming complexes… read more here.
Keywords: exclusion chromatography; analyze; size exclusion; method ... See more keywords
Use of Raman spectroscopy and size-exclusion chromatography coupled with HDX-MS spectroscopy for studying conformational changes of small proteins in solution.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of pharmaceutical and biomedical analysis"
DOI: 10.1016/j.jpba.2020.113399
Abstract: Protein-based drugs are a relatively new paradigm in modern therapeutics. These large molecule drugs often have much higher specificity and selectivity compared to small molecules that have been used in therapeutics for centuries. However, there… read more here.
Keywords: spectroscopy size; exclusion chromatography; solution; spectroscopy ... See more keywords