Fusion and secretory expression of an exo-inulinase and a d-allulose 3-epimerase to produce D-allulose syrup from inulin.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of the science of food and agriculture"
DOI: 10.1002/jsfa.10682
Abstract: BACKGROUND This study developed a feasible catalytic method for d-allulose syrup production using a fusion enzyme, either in free or immobilized form, through hydrolysis of inulin extracted from Jerusalem artichoke tubers. RESULTS d-Allulose 3-epimerase (DAE)… read more here.
Keywords: exo inulinase; inulin; allulose syrup; fusion enzyme ... See more keywords
Removal of N-terminal tail changes the thermostability of the low-temperature-active exo-inulinase InuAGN25
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DOI: 10.1080/21655979.2020.1809921
Abstract: ABSTRACT Exo-inulinases are members of the glycoside hydrolase family 32 and function by hydrolyzing inulin into fructose with yields up to 90–95%. The N-terminal tail contributes to enzyme thermotolerance, which plays an important role in… read more here.
Keywords: inulinase inuagn25; terminal tail; exo inulinase; tail ... See more keywords
Deletion of the Loop Linking Two Domains of Exo-Inulinase InuAMN8 Diminished the Enzymatic Thermo-Halo-Alcohol Tolerance
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.924447
Abstract: Inulin is the rich water-soluble storage polysaccharide after starch in nature, and utilization of inulin through hydrolysis of exo-inulinases has attracted much attention. Thermo-halo-alcohol tolerance is essential for exo-inulinase applications, while no report reveals the… read more here.
Keywords: alcohol tolerance; halo alcohol; exo inulinase; thermo halo ... See more keywords