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   Articles with "exonuclease" as a keyword



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Low frequency of POLD1 and POLE exonuclease domain variants in patients with multiple colorectal polyps

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recommendations! Published in 2019 at "Molecular Genetics & Genomic Medicine"

DOI: 10.1002/mgg3.603

Abstract: Germline mutations affecting the exonuclease domains of POLE and POLD1 predispose to colorectal adenomas and carcinoma. Here, we aimed to screen the exonuclease domains to find the genetic causes of multiple colorectal polyps in unexplained… read more here.

Keywords: exonuclease; colorectal polyps; low frequency; multiple colorectal ... See more keywords
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Ratiometric fluorescence sensor based on carbon dots as internal reference signal and T7 exonuclease-assisted signal amplification strategy for microRNA-21 detection.

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recommendations! Published in 2020 at "Analytica chimica acta"

DOI: 10.1016/j.aca.2019.12.068

Abstract: The expression level of miRNA-21 is closely related to the occurrence and development of cancer, especially in gastrointestinal cancer. Monitoring miRNA-21 has clinical application in the diagnosis and evaluation of gastrointestinal cancer. A turn-on ratiometric… read more here.

Keywords: fluorescence; ratiometric fluorescence; carbon dots; exonuclease ... See more keywords
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RecBCD (Exonuclease V) is inhibited by DNA adducts produced by cisplatin and ultraviolet light.

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recommendations! Published in 2018 at "Biochemical and biophysical research communications"

DOI: 10.1016/j.bbrc.2017.11.060

Abstract: The presence of adducts on the DNA double-helix can have major consequences for the efficient functioning of DNA repair enzymes. E. coli RecBCD (exonuclease V) is involved in recombinational repair of double-strand breaks that are caused… read more here.

Keywords: inhibited dna; dna; exonuclease; recbcd exonuclease ... See more keywords
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Characterization of TK1646, a highly thermostable 3'-5' single strand specific exonuclease from Thermococcus kodakarensis.

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recommendations! Published in 2019 at "International journal of biological macromolecules"

DOI: 10.1016/j.ijbiomac.2019.08.150

Abstract: Exonucleases catalyze the hydrolysis of terminal phosphodiester bond in nucleic acid. They play important role in maintaining the integrity of DNA in eukaryotes, prokaryotes and archaea. Limited studies have been done on archaeal exonucleases. Here… read more here.

Keywords: strand specific; single strand; exonuclease; specific exonuclease ... See more keywords

Biochemical and functional characterization of a thermostable RecJ exonuclease from Thermococcus gammatolerans.

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recommendations! Published in 2022 at "International journal of biological macromolecules"

DOI: 10.1016/j.ijbiomac.2022.02.033

Abstract: RecJ is ubiquitous in bacteria and Archaea, and play an important role in DNA replication and repair. Currently, our understanding on biochemical function of archaeal RecJ is incomplete due to limited reports. The genome of… read more here.

Keywords: tga recj; exonuclease; recj; thermococcus gammatolerans ... See more keywords

Quantification of intrahepatic cccDNA in HBV associated hepatocellular carcinoma by improved ddPCR method.

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recommendations! Published in 2021 at "Journal of virological methods"

DOI: 10.1016/j.jviromet.2021.114334

Abstract: The quantification of intrahepatic covalently closed circular DNA (cccDNA) is important for assessing the efficiency of anti-HBV therapy. Exonuclease treatment is essential before real-time quantitative PCR (qPCR) or droplet digital PCR (ddPCR) measurement to improve… read more here.

Keywords: cccdna; intrahepatic cccdna; exonuclease; quantification ... See more keywords
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RecJ 5' Exonuclease Digestion of Oligonucleotide Failure Strands: A "Green" Method of Trityl-On Purification.

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recommendations! Published in 2017 at "Biochemistry"

DOI: 10.1021/acs.biochem.7b00010

Abstract: Methods of error filtration and correction post-gene assembly are a major bottleneck in the synthetic biology pipeline. Current oligonucleotide purification strategies, including polyacrylamide gel electrophoresis and high-performance liquid chromatography, are often expensive and labor-intensive, give… read more here.

Keywords: trityl purification; exonuclease; purification; failure strands ... See more keywords
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Mechanistic decoupling of exonuclease III multifunctionality into AP endonuclease and exonuclease activities at the single-residue level

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recommendations! Published in 2022 at "Nucleic Acids Research"

DOI: 10.1093/nar/gkac043

Abstract: Abstract Bacterial exonuclease III (ExoIII) is a multifunctional enzyme that uses a single active site to perform two conspicuous activities: (i) apurinic/apyrimidinic (AP)-endonuclease and (ii) 3′→5′ exonuclease activities. The AP endonuclease activity results in AP… read more here.

Keywords: exonuclease activities; exonuclease; endonuclease exonuclease; level ... See more keywords
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Yeast Dxo1 is required for 25S rRNA maturation and acts as a transcriptome-wide distributive exonuclease

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recommendations! Published in 2022 at "RNA"

DOI: 10.1261/rna.078952.121

Abstract: The Dxo1/Rai1/DXO family of decapping and exonuclease enzymes can catalyze the in vitro removal of chemically diverse 5′ ends from RNA. Specifically, these enzymes act poorly on RNAs with a canonical 7mGpppN cap, but instead… read more here.

Keywords: dxo1 required; 25s rrna; yeast dxo1; dxo1 ... See more keywords
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Human Mitochondrial DNA Polymerase Metal Dependent UV Lesion Bypassing Ability

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recommendations! Published in 2022 at "Frontiers in Molecular Biosciences"

DOI: 10.3389/fmolb.2022.808036

Abstract: Human mitochondrial DNA contains more UV-induced lesions than the nuclear DNA due to lack of mechanism to remove bulky photoproducts. Human DNA polymerase gamma (Pol γ) is the sole DNA replicase in mitochondria, which contains… read more here.

Keywords: dna; human mitochondrial; mitochondrial dna; lesion ... See more keywords
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Characterization of hotspot exonuclease domain mutations in the DNA polymerase ϵ gene in endometrial cancer

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recommendations! Published in 2022 at "Frontiers in Oncology"

DOI: 10.3389/fonc.2022.1018034

Abstract: Objective This study was aimed to profile hotspot exonuclease domain mutations (EDMs) of the DNA polymerase ϵ gene (POLE) in endometrial cancer (EC) and to investigate the effects of EDMs on tumor cell behavior and… read more here.

Keywords: endometrial cancer; hotspot; hotspot exonuclease; exonuclease domain ... See more keywords