Trypanosoma brucei Mitochondrial DNA Polymerase POLIB Contains a Novel Polymerase Domain Insertion That Confers Dominant Exonuclease Activity.
Sign Up to like & getrecommendations! Published in 2022 at "Biochemistry"
DOI: 10.1021/acs.biochem.2c00392
Abstract: Trypanosoma brucei and related parasites contain an unusual catenated mitochondrial genome known as kinetoplast DNA (kDNA) composed of maxicircles and minicircles. The kDNA structure and replication mechanism are divergent and essential for parasite survival. POLIB… read more here.
Keywords: polymerase; dna; trypanosoma brucei; polib ... See more keywords
A 3′-5′ exonuclease activity embedded in the helicase core domain of Candida albicans Pif1 helicase
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/srep42865
Abstract: 3′-5′ exonucleases are frequently found to be associated to polymerases or helicases domains in the same enzyme or could function as autonomous entities. Here we uncovered that Candida albicans Pif1 (CaPif1) displays a 3′-5′ exonuclease… read more here.
Keywords: candida albicans; helicase; activity; domain ... See more keywords
A novel fluorescent assay for uracil DNA glycosylase activity built on the 3′–5′ exonuclease activity-based endonuclease IV cyclic signal amplification strategy
Sign Up to like & getrecommendations! Published in 2020 at "New Journal of Chemistry"
DOI: 10.1039/d0nj04729e
Abstract: The unique 3′-5′ exonuclease activity of endonuclease IV to DNA strands has been demonstrated, which enables the development of a novel highly sensitive assay for UDG activity. read more here.
Keywords: endonuclease; novel fluorescent; activity; fluorescent assay ... See more keywords
RNA substrate length as an indicator of exosome interactions in vivo
Sign Up to like & getrecommendations! Published in 2017 at "Wellcome Open Research"
DOI: 10.12688/wellcomeopenres.10724.2
Abstract: Background: The exosome complex plays key roles in RNA processing and degradation in Eukaryotes and Archaea. Outstanding structural studies identified multiple pathways for RNA substrates into the exosome in vitro, but identifying the pathway followed… read more here.
Keywords: length; vivo rna; substrate length; rna substrate ... See more keywords