Photoelectrochemical aptasensing for thrombin based on exonuclease III-assisted recycling signal amplification and nanoceria enzymatic strategy.
Sign Up to like & getrecommendations! Published in 2021 at "Talanta"
DOI: 10.1016/j.talanta.2021.122577
Abstract: In the present work, a capture DNA (c-DNA) was immobilized on the TNA/g-C3N4 to develop a sensitive and selective TNA/g-C3N4/c-DNA photoelectrochemical aptasensor for determining thrombin. With the aid of the specific recognition of anti-thrombin aptamer… read more here.
Keywords: iii assisted; dna; tna c3n4; exonuclease iii ... See more keywords
Self-Cleaning Recyclable Multiplexed Photoelectrochemical Sensing Strategy Based on Exonuclease III-Assisted Signal Discrimination.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.3c01012
Abstract: For discriminating the signals of multi-targets, multiplexed photoelectrochemical (PEC) detection is generally accomplished by modulating the light source or voltage, which prospect is usually limited by expensive instrumentation, tedious operational steps, and time-consuming material screening.… read more here.
Keywords: exonuclease iii; strategy; multiplexed photoelectrochemical; signal discrimination ... See more keywords
A simple and rapid dual-cycle amplification strategy for microRNA based on graphene oxide and exonuclease III-assisted fluorescence recovery
Sign Up to like & getrecommendations! Published in 2018 at "Analytical Methods"
DOI: 10.1039/c8ay01106k
Abstract: A simple microRNA detection method by combining Graphene Oxide (GO) fluorescence quenching with exonuclease III (Exo-III) aided cycling amplification was developed. Three DNA probes, a FAM-containing ssDNA (P-DNA), hairpin probe 1 (H1) and hairpin probe… read more here.
Keywords: graphene oxide; iii; fluorescence; exonuclease iii ... See more keywords
Mechanistic decoupling of exonuclease III multifunctionality into AP endonuclease and exonuclease activities at the single-residue level
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac043
Abstract: Abstract Bacterial exonuclease III (ExoIII) is a multifunctional enzyme that uses a single active site to perform two conspicuous activities: (i) apurinic/apyrimidinic (AP)-endonuclease and (ii) 3′→5′ exonuclease activities. The AP endonuclease activity results in AP… read more here.
Keywords: exonuclease activities; exonuclease; endonuclease exonuclease; level ... See more keywords
Label-free rapid and simple detection of exonuclease III Activity with DNA Templated Copper Nanoclusters.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of microbiology and biotechnology"
DOI: 10.4014/jmb.1804.04060
Abstract: In this study, DNA-templated copper nanoclusters (DNA-CuNCs) were used to detect exonuclease III (Exo III) activity, which is important for the diagnosis and therapy of several diseases. The results of this study showed that Exo… read more here.
Keywords: iii; dna; templated copper; copper nanoclusters ... See more keywords