Challenges of Using Expansion Microscopy for Super‐resolved Imaging of Cellular Organelles
Sign Up to like & getrecommendations! Published in 2020 at "Chembiochem"
DOI: 10.1002/cbic.202000571
Abstract: Expansion microscopy (ExM) has been successfully used to improve the spatial resolution when imaging tissues by optical microscopy. In ExM, proteins of a fixed sample are crosslinked to a swellable acrylamide gel, which expands when… read more here.
Keywords: expansion; microscopy; expansion factors; using expansion ... See more keywords
Expansion Microscopy of Larval Zebrafish Brains and Zebrafish Embryos.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2051-9_13
Abstract: Since its introduction in 2015, expansion microscopy (ExM) allowed imaging a broad variety of biological structures in many models, at nanoscale resolution. Here, we describe in detail a protocol for application of ExM in whole-brains… read more here.
Keywords: brains zebrafish; microscopy; microscopy larval; larval zebrafish ... See more keywords
Nanoscale imaging using differential expansion microscopy
Sign Up to like & getrecommendations! Published in 2020 at "Histochemistry and Cell Biology"
DOI: 10.1007/s00418-020-01869-7
Abstract: Expensive and time-consuming approaches of immunoelectron microscopy of biopsy tissues continues to serve as the gold-standard for diagnostic pathology. The recent development of the new approach of expansion microscopy (ExM) capable of fourfold lateral expansion… read more here.
Keywords: differential expansion; expansion; microscopy; imaging using ... See more keywords
Enhanced expansion microscopy to measure nanoscale structural and biochemical remodeling in single cells.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2020.04.019
Abstract: Resolution is a key feature in microscopy which allows the visualization of the fine structure of cells. Much of the life processes within these cells depend on the three-dimensional (3D) complexity of these structures. Optical… read more here.
Keywords: resolution; microscopy; super resolution; expansion microscopy ... See more keywords
Protein-retention expansion microscopy for visualizing subcellular organelles in fixed brain tissue
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Neuroscience Methods"
DOI: 10.1016/j.jneumeth.2021.109285
Abstract: BACKGROUND Protein expansion microscopy (proExM) is a powerful technique that crosslinks proteins to a swellable hydrogel to physically expand and optically clear biological samples. The resulting increased resolution (~70nm) and physical separation of labeled proteins… read more here.
Keywords: microscopy; visualizing subcellular; brain; expansion microscopy ... See more keywords
Current Progress in Expansion Microscopy: Chemical Strategies and Applications.
Sign Up to like & getrecommendations! Published in 2023 at "Chemical reviews"
DOI: 10.1021/acs.chemrev.2c00711
Abstract: Expansion microscopy (ExM) is a newly developed super-resolution technique, allowing visualization of biological targets at nanoscale resolution on conventional fluorescence microscopes. Since its introduction in 2015, many efforts have been dedicated to broaden its application… read more here.
Keywords: expansion microscopy; current progress; microscopy; resolution ... See more keywords
Meeting Zn Needs during Medaka Eye Development: Nanoscale Visualization of Retina by Expansion Microscopy.
Sign Up to like & getrecommendations! Published in 2022 at "Environmental science & technology"
DOI: 10.1021/acs.est.2c06479
Abstract: Fish eyes require high Zn levels to support their early development. Although numerous studies have been conducted on the nutritional and toxic effects of Zn on the eye, the Zn requirement for retinal cell development… read more here.
Keywords: expansion microscopy; cell; development; microscopy ... See more keywords
Expansion Microscopy for Imaging the Cell-Material Interface.
Sign Up to like & getrecommendations! Published in 2022 at "ACS nano"
DOI: 10.1021/acsnano.1c11015
Abstract: Surface topography on the scale of tens of nanometers to several micrometers substantially affects cell adhesion, migration, and differentiation. Recent studies using electron microscopy and super-resolution microscopy provide insight into how cells interact with surface… read more here.
Keywords: expansion microscopy; cell; microscopy; cell material ... See more keywords
Lipid Expansion Microscopy.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c03743
Abstract: Strategies to visualize cellular membranes with light microscopy are restricted by the diffraction limit of light, which far exceeds the dimensions of lipid bilayers. Here, we describe a method for super-resolution imaging of metabolically labeled… read more here.
Keywords: expansion microscopy; cellular membranes; microscopy; super resolution ... See more keywords
Nanoscale imaging of bacterial infections by sphingolipid expansion microscopy
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-19897-1
Abstract: Expansion microscopy (ExM) enables super-resolution imaging of proteins and nucleic acids on conventional microscopes. However, imaging of details of the organization of lipid bilayers by light microscopy remains challenging. We introduce an unnatural short-chain azide-… read more here.
Keywords: expansion; microscopy; imaging bacterial; nanoscale imaging ... See more keywords
Volumetric, Nanoscale Optical Imaging of Mouse and Human Kidney via Expansion Microscopy
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-28694-2
Abstract: Although light microscopy is a powerful tool for the assessment of kidney physiology and pathology, it has traditionally been unable to resolve structures separated by less than the ~250 nm diffraction limit of visible light. Here,… read more here.
Keywords: microscopy; volumetric nanoscale; mouse human; human kidney ... See more keywords