Exploring sequence space in search of functional enzymes using microfluidic droplets.
Sign Up to like & getrecommendations! Published in 2017 at "Current opinion in chemical biology"
DOI: 10.1016/j.cbpa.2017.02.018
Abstract: Screening of enzyme mutants in monodisperse picoliter compartments, generated at kilohertz speed in microfluidic devices, is coming of age. After a decade of proof-of-principle experiments, workflows have emerged that combine existing microfluidic modules to assay… read more here.
Keywords: sequence space; exploring sequence; search functional; space search ... See more keywords
Exploring the sequence fitness landscape of a bridge between protein folds
Sign Up to like & getrecommendations! Published in 2020 at "PLoS Computational Biology"
DOI: 10.1371/journal.pcbi.1008285
Abstract: Most foldable protein sequences adopt only a single native fold. Recent protein design studies have, however, created protein sequences which fold into different structures apon changes of environment, or single point mutation, the best characterized… read more here.
Keywords: protein; exploring sequence; fitness landscape;