Gene Expression Analysis of T-Cells by Single-Cell RNA-Seq.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1311-5_22
Abstract: During the last decade, the rapid progress in the development of next-generation sequencing (NGS) technologies has provided relevant insights into complex biological systems, ranging from cancer genomics to microbiology. Among NGS technologies, single-cell RNA sequencing… read more here.
Keywords: single cell; gene expression; cell rna; expression analysis ... See more keywords
In Planta Gene Expression Analysis and Colonization of Fusarium oxysporum.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1795-3_12
Abstract: In planta gene expression analysis and GFP-based confocal microscopy are two powerful techniques that may be coupled to assess the extent and dynamics of plant colonization by a fungal pathogen. Here we describe methods to… read more here.
Keywords: fusarium oxysporum; microscopy; gene expression; colonization ... See more keywords
MicroRNA Expression Analysis Using Small RNA Sequencing Discovery and RT-qPCR-Based Validation.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7231-9_13
Abstract: miRNAs are small noncoding RNA molecules that function as regulators of gene expression. Deregulated miRNA expression has been reported in various diseases including cancer. Due to their small size and high degree of homology, accurate… read more here.
Keywords: small rna; microrna expression; rna sequencing; expression ... See more keywords
Semiautomated Small-Scale Purification Method for High-Throughput Expression Analysis of Recombinant Proteins.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9624-7_3
Abstract: The expression analysis of recombinant proteins is a challenging step in any high-throughput protein production pipeline. Often multiple expression systems and a variety of expression construct designs are considered for the production of a protein… read more here.
Keywords: analysis recombinant; expression; expression analysis; recombinant proteins ... See more keywords
Expression analysis of defense-related genes in cucumber (Cucumis sativus L.) against Phytophthora melonis
Sign Up to like & getrecommendations! Published in 2020 at "Molecular Biology Reports"
DOI: 10.1007/s11033-020-05520-5
Abstract: Phytophthora melonis is one of the most destructive cucumber disease, causing severe economic losses in the globe. Despite intense research efforts made in the past years, no permanent cure currently exists for this disease. With… read more here.
Keywords: melonis; defense; expression; phytophthora melonis ... See more keywords
Identification and expression analysis of microRNAs in tibial growth plate of chicken through thiram toxicity
Sign Up to like & getrecommendations! Published in 2019 at "Environmental Science and Pollution Research"
DOI: 10.1007/s11356-019-06648-z
Abstract: Thiram is a widely known tibial dyschondroplasia (TD) inducer. TD, a common metabolic cartilage disease, presents in rapidly growing poultry birds. There are evidences that miRNAs are involved in diverse aspects of normal skeletal development,… read more here.
Keywords: analysis; identification expression; expression; expression analysis ... See more keywords
Characterization and expression analysis of genes encoding Taxol biosynthetic enzymes in Taxus spp.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Forestry Research"
DOI: 10.1007/s11676-021-01290-3
Abstract: Taxol (Paclitaxel), an important anticancer drug, is derived at very low yields from Taxus (yew) species that grow very slowly. In the present study, thirteen genes that encode enzymes involved in Taxol biosynthesis in Taxus… read more here.
Keywords: expression analysis; taxus spp; taxol; characterization expression ... See more keywords
Isolation and expression analysis of EcbZIP17 from different finger millet genotypes shows conserved nature of the gene
Sign Up to like & getrecommendations! Published in 2017 at "3 Biotech"
DOI: 10.1007/s13205-017-0984-2
Abstract: Basic leucine zipper (bZIP) transcription factors comprise one of the largest gene families in plants. They play a key role in almost every aspect of plant growth and development and also in biotic and abiotic… read more here.
Keywords: expression; analysis ecbzip17; expression analysis; finger millet ... See more keywords
Transcriptome wide identification and characterization of regulatory genes involved in EAA metabolism and validation through expression analysis in different developmental stages of finger millet spikes
Sign Up to like & getrecommendations! Published in 2020 at "3 Biotech"
DOI: 10.1007/s13205-020-02337-8
Abstract: Finger millet is a rich source of seed storage proteins (SSPs). Various regulatory genes play an important role to maintain the quality and accumulation of SSPs in crop seeds. In the present study, nine regulatory… read more here.
Keywords: synthase; regulatory genes; analysis; expression analysis ... See more keywords
Expression analysis of chitinase upon challenge inoculation to Alternaria wounding and defense inducers in Brassica juncea
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology Reports"
DOI: 10.1016/j.btre.2017.01.001
Abstract: Highlights • Expression of chitinase gene was studied by RT-PCR in response to Alternaria brassicae.• Chitinase gene is induced by Alternaria, wounding and by JA and not by SA. It shows the tissue specificity of… read more here.
Keywords: expression analysis; alternaria wounding; chitinase gene; chitinase ... See more keywords
Comparison of Methods for Differential Co-expression Analysis for Disease Biomarker Prediction
Sign Up to like & getrecommendations! Published in 2019 at "Computers in biology and medicine"
DOI: 10.1016/j.compbiomed.2019.103380
Abstract: In the recent past, a number of methods have been developed for analysis of biological data. Among these methods, gene co-expression networks have the ability to mine functionally related genes with similar co-expression patterns, because… read more here.
Keywords: differential expression; analysis; comparison methods; disease ... See more keywords