Assessment of Cre-lox and CRISPR-Cas9 as tools for recycling of multiple-integrated selection markers in Saccharomyces cerevisiae
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Microbiology"
DOI: 10.1007/s12275-022-1580-7
Abstract: We evaluated the Cre-lox and CRISPR-Cas9 systems as marker-recycling tools in Saccharomyces cerevisiae recombinants containing multiple-integrated expression cassettes. As an initial trial, we constructed rDNA-nontranscribed spacer- or Ty4-based multiple integration vectors containing the URA3 marker… read more here.
Keywords: marker; expression cassettes; crispr cas9; cre lox ... See more keywords
Directed expression of halophilic and acidophilic β-glucosidases by introducing homologous constitutive expression cassettes in marine Aspergillus niger.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2018.12.015
Abstract: The hydrolysis step by β-glucosidase (BGL) is generally recognized as the major limiting step in cellulose degradation and the BGLs with prominent enzymatic properties are of great importance for efficient utilization of lignocellulosic biomass. In… read more here.
Keywords: aspergillus niger; directed expression; expression cassettes; expression ... See more keywords
An Efficient Modular Gateway Recombinase-Based Gene Stacking System for Generating Multi-Trait Transgenic Plants
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DOI: 10.3390/plants11040488
Abstract: Transgenic technology can transfer favorable traits regardless of reproductive isolation and is an important method in plant synthetic biology and genetic improvement. Complex metabolic pathway modification and pyramiding breeding strategies often require the introduction of… read more here.
Keywords: gene stacking; expression cassettes; gene; stacking system ... See more keywords
Bidirectional promoter activity from expression cassettes can drive off-target repression of neighboring gene translation
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.7554/elife.81086
Abstract: Targeted selection-based genome-editing approaches have enabled many fundamental discoveries and are used routinely with high precision. We found, however, that replacement of DBP1 with a common selection cassette in budding yeast led to reduced expression… read more here.
Keywords: cassette; neighboring gene; expression; expression cassettes ... See more keywords