Gene cloning, expression, and characterization of a novel β‐mannanase from the endophyte Paenibacillus sp. CH‐3
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Applied Biochemistry"
DOI: 10.1002/bab.1510
Abstract: An endophyte with high yield of β‐mannanase, designated as Paenibacillus sp. CH‐3, was isolated from black locust (Robinia pseudoacacia) seeds. The β‐mannanase gene (manB) has been cloned, expressed, purified, and characterized. With an open reading… read more here.
Keywords: expression characterization; mannanase; manb; gene cloning ... See more keywords
Expression and characterization of chimeric spidroins from flagelliform‐aciniform repetitive modules
Sign Up to like & getrecommendations! Published in 2020 at "Biopolymers"
DOI: 10.1002/bip.23404
Abstract: Spiders can produce up to seven different types of silks or glues with different mechanical properties. Of these, flagelliform (Flag) silk is the most elastic, and aciniform (AcSp1) silk is the toughest. To produce a… read more here.
Keywords: expression characterization; flagr acsp1r; chimeric; aciniform ... See more keywords
Identification, expression and characterization of an R-ω-transaminase from Capronia semiimmersa
Sign Up to like & getrecommendations! Published in 2017 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-017-8309-2
Abstract: Chiral amines are essential precursors in the production of biologically active compounds, including several important drugs. Among the biocatalytic strategies that have been developed for their synthesis, the use of ω-transaminases (ω-TA) appears as an… read more here.
Keywords: expression characterization; characterization transaminase; identification expression; capronia semiimmersa ... See more keywords
Expression and characterization of rainbow trout Oncorhynchus mykiss recombinant myoglobin.
Sign Up to like & getrecommendations! Published in 2021 at "Fish physiology and biochemistry"
DOI: 10.1007/s10695-021-00991-0
Abstract: Recombinant expression system was established for rainbow trout myoglobin (Mb) considering its unique primary structure of having one unusual deletion and two cysteine residues in contrast to the other fish Mbs. The obtained recombinant Mb… read more here.
Keywords: expression characterization; recombinant; myoglobin; rainbow trout ... See more keywords
Homologous Expression and Characterization of α-L-rhamnosidase from Aspergillus niger for the Transformation of Flavonoids
Sign Up to like & getrecommendations! Published in 2022 at "Applied Biochemistry and Biotechnology"
DOI: 10.1007/s12010-022-03894-9
Abstract: Aspergillus niger has been used for homologous and heterologous expressions of many protein products. In this study, the α-L-rhamnosidase from A. niger (Rha-N1, GenBank XP_001389086.1) was homologously expressed in A. niger 3.350 by Agrobacterium tumefaciens-mediated… read more here.
Keywords: aspergillus niger; characterization rhamnosidase; homologous expression; transformation ... See more keywords
Expression and Characterization of Glucose Oxidase from Aspergillus niger in Yarrowia lipolytica
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Biotechnology"
DOI: 10.1007/s12033-017-0017-8
Abstract: Glucose oxidase (GOX) is currently used in clinical, pharmaceutical, food and chemical industries. The aim of this study was expression and characterization of Aspergillus niger glucose oxidase gene in the yeast Yarrowia lipolytica. For the… read more here.
Keywords: oxidase; expression characterization; glucose oxidase; niger ... See more keywords
Cloning, Expression, and Characterization of a Thermotolerant β-agarase from Simiduia sp. SH-4
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioprocess Engineering"
DOI: 10.1007/s12257-018-0072-4
Abstract: The gene coding for a thermotolerant β-agarase from an isolated Simiduia sp. SH-4 was cloned, recombinantly expressed, and characterized after purification. This gene was sequenced after cassette mediated polymerase chain reaction and composed of an… read more here.
Keywords: agarase; expression characterization; agarase simiduia; thermotolerant agarase ... See more keywords
Cloning, expression, and characterization of a novel superoxide dismutase from deep-sea sea cucumber.
Sign Up to like & getrecommendations! Published in 2020 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2020.09.135
Abstract: Superoxide dismutases (SODs) are the first line of defense against oxidative damage caused by reactive oxygen species and are associated with stress tolerance. Accordingly, many studies have focused on SODs, especially those from extreme habitats.… read more here.
Keywords: characterization novel; deep sea; expression characterization; sea ... See more keywords
Expression and characterization of the benzoic acid hydroxylase CYP199A25 from Arthrobacter sp.
Sign Up to like & getrecommendations! Published in 2020 at "Molecular Catalysis"
DOI: 10.1016/j.mcat.2019.110739
Abstract: Abstract Metabolically diverse organisms can serve as valuable source for Cytochrome P450 monooxygenases (CYPs), extending the repertoire of biocatalysts for C–H bond oxyfunctionalization. Here, we report on the cloning, expression and characterization of two new P450 s… read more here.
Keywords: acid hydroxylase; expression characterization; cyp199a25; benzoic acid ... See more keywords
Expression and characterization of a codon‐optimized blood coagulation factor VIII
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Thrombosis and Haemostasis"
DOI: 10.1111/jth.13632
Abstract: Essentials Recombinant factor VIII (FVIII) is known to be expressed at a low level in cell culture. To increase expression, we used codon‐optimization of a B‐domain deleted FVIII (BDD‐FVIII). This resulted in 7‐fold increase of… read more here.
Keywords: codon optimized; expression characterization; factor viii; characterization codon ... See more keywords
Cloning, expression and characterization of metalloproteinase HypZn from Aspergillus niger
Sign Up to like & getrecommendations! Published in 2021 at "PLoS ONE"
DOI: 10.1371/journal.pone.0259809
Abstract: A predicted metalloproteinase gene, HypZn, was cloned from Aspergillus niger CGMCC 3.7193 and expressed in Pichia pastoris GS115, and the physicochemical characteristics of recombinant HypZn were investigated after separation and purification. The results showed that… read more here.
Keywords: aspergillus niger; hypzn; expression characterization; metalloproteinase ... See more keywords