Increased expression of the fluorescent reporter protein ymNeonGreen in Saccharomyces cerevisiae by reducing RNA secondary structure near the start codon.
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DOI: 10.1016/j.btre.2021.e00697
Abstract: Highlights • Stable secondary RNA structure 3′ of the start codon inhibits ymNeonGreen expression.• Removing secondary RNA structure increased expression in S. cerevisiae and E. coli.• Expression was increased 2-fold in S. cerevisiae.• Expression was… read more here.
Keywords: fluorescent reporter; expression; start codon; increased expression ... See more keywords