Toward tunable dynamic repression using CRISPRi.
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology journal"
DOI: 10.1002/biot.201800152
Abstract: CRISPR interference (CRISPRi) is widely utilized for regulation of target gene expression by repressing transcription. Simple design rules for the single guide RNA (sgRNA) and multiplexity won this method immense popularity. However, quantitative control of… read more here.
Keywords: toward tunable; expression level; using crispri; expression ... See more keywords
Plasmid expression level heterogeneity monitoring via heterologous eGFP production at the single-cell level in Cupriavidus necator
Sign Up to like & getrecommendations! Published in 2020 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-020-10616-w
Abstract: A methodology for plasmid expression level monitoring of eGFP expression suitable for dynamic processes was assessed during fermentation. This technique was based on the expression of a fluorescent biosensor (eGFP) encoded on a recombinant plasmid… read more here.
Keywords: single cell; expression level; level; plasmid expression ... See more keywords
Expressions and significances of CTSL, the target of COVID‐19 on GBM
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Cancer Research and Clinical Oncology"
DOI: 10.1007/s00432-021-03843-9
Abstract: Cathepsin L (CTSL) is a kind of the SARS-entry-associated CoV-2's proteases, which plays a key role in the virus's entry into the cell and subsequent infection. We investigated the association between the expression level of… read more here.
Keywords: expression level; gbm; target; level ctsl ... See more keywords
Codon usage and amino acid usage influence genes expression level
Sign Up to like & getrecommendations! Published in 2017 at "Genetica"
DOI: 10.1007/s10709-017-9996-4
Abstract: Highly expressed genes in any species differ in the usage frequency of synonymous codons. The relative recurrence of an event of the favored codon pair (amino acid pairs) varies between gene and genomes due to… read more here.
Keywords: amino; gene; expression level; amino acid ... See more keywords
Cisplatin promotes the expression level of PD-L1 in the microenvironment of hepatocellular carcinoma through YAP1
Sign Up to like & getrecommendations! Published in 2020 at "Molecular and Cellular Biochemistry"
DOI: 10.1007/s11010-020-03861-0
Abstract: Hepatocellular carcinoma (HCC) is one of the most lethal malignancies worldwide. However, the immune tolerance limits the effect of chemotherapeutic drugs. Therefore, the mechanism of cisplatin in promoting PD-L1 expression by YAP1 was investigated in… read more here.
Keywords: cisplatin; microenvironment; yap1; expression level ... See more keywords
Study of Vesicular Monoamine Transporter 2 in Myopic Retina Using [18F]FP-(+)-DTBZ
Sign Up to like & getrecommendations! Published in 2018 at "Molecular Imaging and Biology"
DOI: 10.1007/s11307-018-1183-1
Abstract: PurposeTo investigate the relationship between expression level of vesicular monoamine transporter 2 (VMAT2) and myopia, as well as the feasibility of noninvasive myopia diagnosis through imaging VMAT2 in retina by using [18F]fluoropropyl-(+)-dihydrotetrabenazine ([18F]FP-(+)-DTBZ).ProceduresThe right eyes… read more here.
Keywords: expression level; using 18f; 18f dtbz; retina ... See more keywords
Marginal Zinc Deficiency in Mice Increased the Number of Abnormal Sperm and Altered the Expression Level of Spermatogenesis-Related Genes.
Sign Up to like & getrecommendations! Published in 2021 at "Biological trace element research"
DOI: 10.1007/s12011-021-02979-6
Abstract: Marginal zinc deficiency is more common than severe zinc deficiency, and the effect of marginal zinc deficiency on male reproduction is unknown. This study investigated the effect of marginal zinc deficiency on spermatogenesis and its… read more here.
Keywords: zinc deficiency; spermatogenesis; expression level; zinc ... See more keywords
Expression of TSLC1 in patients with HAM/TSP
Sign Up to like & getrecommendations! Published in 2020 at "Journal of NeuroVirology"
DOI: 10.1007/s13365-020-00838-z
Abstract: Human T-lymphotropic virus type 1 (HTLV-1)–associated myelopathy/tropical spastic paraparesis (HAM/TSP) is chronic myelopathy characterized by slowly progressive spastic paraparesis and urinary dysfunction. A few biomarkers in the cerebrospinal fluid are known to be related to… read more here.
Keywords: expression level; tslc1; ham tsp; disease activity ... See more keywords
Sterol regulatory element binding protein-1: Molecular cloning, tissue distribution and gene expression level in response to nutritional regulation in mud crab, Scylla paramamosain.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2018.09.154
Abstract: In the present study, SREBP-1 cDNA was cloned from the hepatopancreas of mud crab (Scylla paramamosain) and characterized by performing rapid-amplification of cDNA ends. The 3361bp long full-length cDNA encodes a polypeptide with 1039 amino… read more here.
Keywords: expression level; scylla paramamosain; tissue distribution; mud crab ... See more keywords
Cross-talk pattern between GABAA- and glycine-receptors in CNS neurons is shaped by their relative expression levels
Sign Up to like & getrecommendations! Published in 2020 at "Brain Research"
DOI: 10.1016/j.brainres.2020.147071
Abstract: GABAA receptors (GABAARs) and glycine receptors (GlyRs) are two principal inhibitory chloride ion channels in the central nervous system. The two receptors do not function independently but cross-talk to each other, i.e., the activation of… read more here.
Keywords: cross talk; expression level; talk; pattern ... See more keywords
Knockdown of DEAD-box RNA helicase DDX5 selectively attenuates serine 311 phosphorylation of NF-κB p65 subunit and expression level of anti-apoptotic factor Bcl-2.
Sign Up to like & getrecommendations! Published in 2019 at "Cellular signalling"
DOI: 10.1016/j.cellsig.2019.109428
Abstract: NF-κB plays a key role in the transcriptional regulation of genes involved in immunity, inflammation, cell proliferation, and oncogenesis. The NF-κB activation process includes nuclear translocation, followed by association with basal transcription machinery. These steps… read more here.
Keywords: expression level; rna helicase; dead box; phosphorylation p65 ... See more keywords