Toward tunable dynamic repression using CRISPRi.
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology journal"
DOI: 10.1002/biot.201800152
Abstract: CRISPR interference (CRISPRi) is widely utilized for regulation of target gene expression by repressing transcription. Simple design rules for the single guide RNA (sgRNA) and multiplexity won this method immense popularity. However, quantitative control of… read more here.
Keywords: toward tunable; expression level; using crispri; expression ... See more keywords
High ENT1 and DCK gene expression levels are a potential biomarker to predict favorable response to nelarabine therapy in T‐cell acute lymphoblastic leukemia
Sign Up to like & getrecommendations! Published in 2019 at "Hematological Oncology"
DOI: 10.1002/hon.2654
Abstract: F IGURE 1 Combined ENT1 and DCK gene expression levels are highly associated with ara-G sensitivity in humanT-ALL cell lines. A, The schema of pharmacological activation pathway of nelarabine. B, Ara-G sensitivity of humanT-ALL cell… read more here.
Keywords: ent1 dck; cell lines; gene expression; expression levels ... See more keywords
Promotion of Monacolin K production in Monascus extractive fermentation: the variation in fungal morphology and in the expression levels of biosynthetic gene clusters.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of the science of food and agriculture"
DOI: 10.1002/jsfa.11218
Abstract: BACKGROUND Monacolin K, an important secondary metabolite of Monascus, possesses a cholesterol-lowering effect, and is widely used in the manufacture of antihypertensive drugs. In this study, we constructed an extractive fermentation system by adding nonionic… read more here.
Keywords: fermentation; morphology; monascus; expression levels ... See more keywords
Associations between expression levels of nucleotide excision repair proteins in lymphoblastoid cells and risk of squamous cell carcinoma of the head and neck
Sign Up to like & getrecommendations! Published in 2018 at "Molecular Carcinogenesis"
DOI: 10.1002/mc.22801
Abstract: Squamous cell carcinoma of head and neck (SCCHN) is one of the most common malignancies worldwide, and nucleotide excision repair (NER) is involved in SCCHN susceptibility. In this analysis of 349 newly diagnosed SCCHN patients… read more here.
Keywords: carcinoma head; cell carcinoma; squamous cell; expression ... See more keywords
Real-Time PCR Analysis of Metabolism-Related Genes in a Long-Lived Model of C. elegans.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0471-7_12
Abstract: In the nematode Caenorhabditis elegans, the mammalian tumor suppressor p53 ortholog CEP-1 (C. elegans p53-like protein) is associated not only with the stress response, germline apoptosis, and meiotic chromosome segregation but also with longevity through the… read more here.
Keywords: real time; pcr; expression levels; gene ... See more keywords
Differences in PD-1 expression on CD8+ T-cells in chronic myeloid leukemia patients according to disease phase and TKI medication
Sign Up to like & getrecommendations! Published in 2020 at "Cancer Immunology, Immunotherapy"
DOI: 10.1007/s00262-020-02617-5
Abstract: Malignant cells can increase in number using immune escape mechanisms such as immune checkpoints. In this study, we evaluated the expression of an immune checkpoint programmed death 1 (PD-1) on T-cell subsets in chronic myeloid… read more here.
Keywords: phase; cd8 cells; expression; expression levels ... See more keywords
Gene expression levels of gamma-glutamyl hydrolase in tumor tissues may be a useful biomarker for the proper use of S-1 and tegafur-uracil/leucovorin in preoperative chemoradiotherapy for patients with rectal cancer
Sign Up to like & getrecommendations! Published in 2017 at "Cancer Chemotherapy and Pharmacology"
DOI: 10.1007/s00280-017-3295-8
Abstract: PurposePreoperative chemoradiotherapy (CRT) using 5-fluorouracil (5-FU)-based chemotherapy is the standard of care for rectal cancer. The effect of additional chemotherapy during the period between the completion of radiotherapy and surgery remains unclear. Predictive factors for… read more here.
Keywords: rectal cancer; response; expression; expression levels ... See more keywords
Identification and functional characterization of three iridoid synthases in Gardenia jasminoides.
Sign Up to like & getrecommendations! Published in 2022 at "Planta"
DOI: 10.1007/s00425-022-03824-3
Abstract: MAIN CONCLUSION The discovery of three iridoid synthases (GjISY, GjISY2 and GjISY4) from Gardenia jasminoides and their functional characterization increase the understanding of iridoid scaffold/iridoid glycoside biosynthesis in iridoid-producing plants. Iridoids are a class of… read more here.
Keywords: iridoid synthases; gardenia jasminoides; expression levels; iridoid ... See more keywords
JAK2 and PTPRC mRNA expression in peripheral blood mononuclear cells from patients with systemic lupus erythematosus
Sign Up to like & getrecommendations! Published in 2019 at "Clinical Rheumatology"
DOI: 10.1007/s10067-019-04778-w
Abstract: In this study, we aimed to explore the expression levels of JAK2 and PTPRC in peripheral blood mononuclear cells (PBMCs) from SLE patients and controls, detect the effects of SLE activity on genes mRNA expression,… read more here.
Keywords: ptprc mrna; jak2 ptprc; expression; expression levels ... See more keywords
The expression of aquaporin-4 is regulated based on innervation in skeletal muscles
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Muscle Research and Cell Motility"
DOI: 10.1007/s10974-018-9494-z
Abstract: Aquaporin-4 (AQP4) is a selective water channel, which expresses on the plasma membrane of myofibers and regulates the osmotic pressure, energy metabolism and morphological changes in myofibers by modulating water transport across sarcolemma in skeletal… read more here.
Keywords: skeletal muscles; innervation skeletal; expression; expression levels ... See more keywords
PYHIN genes as potential biomarkers for prognosis of human papillomavirus-positive or -negative head and neck squamous cell carcinomas
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Biology Reports"
DOI: 10.1007/s11033-019-04795-7
Abstract: The aim of the present study is to determine the expression levels of PYHIN (IFI16 and AIM2) and APOBEC3 (A3A, A3B, A3C, A3D, A3F, A3G, and A3H) gene family members in a cohort of patients… read more here.
Keywords: neck squamous; squamous cell; expression; hpv ... See more keywords