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Published in 2018 at "Journal of Biomolecular NMR"
DOI: 10.1007/s10858-018-0179-0
Abstract: We present protocols for high-level expression of isotope-labelled proteins in E. coli in cost-effective ways. This includes production of large amounts of unlabeled proteins and 13C-methyl methionine labeling in rich media, where yields of up…
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Keywords:
large scale;
scale expression;
expression proteins;
effective large ... See more keywords
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Published in 2023 at "PLOS ONE"
DOI: 10.1371/journal.pone.0283592
Abstract: The emergence of COVID-19 as a global pandemic had sharply illustrated the limitations of research and development pipelines and scaled manufacturing. Although existing vaccines were created in record time, global deployment remains limited by regional…
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Keywords:
proteins relevant;
production;
thraustochytrid hosts;
hosts expression ... See more keywords
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Published in 2020 at "Frontiers in Veterinary Science"
DOI: 10.3389/fvets.2020.612130
Abstract: Background: Pulmonary hypertension (PH) can cause medial thickening, a hallmark of pulmonary arterial remodeling. The serotonin (5HT) pathway has been suggested as a factor associated with PH by inducing pulmonary arterial smooth muscle cells (SMCs)…
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Keywords:
dmvd;
expression proteins;
5ht pathway;
proteins related ... See more keywords
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Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23105366
Abstract: Autoimmune lymphoproliferative syndrome (ALPS) is a rare disease defined as a defect in the lymphocyte apoptotic pathway. Currently, the diagnosis of ALPS is based on clinical aspects, defective lymphocyte apoptosis and mutations in Fas, FasL…
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Keywords:
cell cycle;
lymphoproliferative syndrome;
differential expression;
autoimmune lymphoproliferative ... See more keywords