A Single Common Protocol for the Expression and Purification of Soluble Mammalian DSPs from Escherichia coli.
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DOI: 10.1007/978-1-0716-0676-6_3
Abstract: Mammalian DSPs have been historically isolated either from native tissue sources or from transfected insect cell cultures via time-consuming and cumbersome protocols often yielding protein of variable quality and quantity. A facile and highly reproducible… read more here.
Keywords: soluble mammalian; expression purification; mammalian dsps; dsps ... See more keywords
Expression and Purification of Human Mitochondrial Intramembrane Protease PARL.
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DOI: 10.1007/978-1-0716-1394-8_1
Abstract: Rhomboid proteases are a ubiquitous superfamily of serine intramembrane peptidases that play a role in a wide variety of cellular processes. The mammalian mitochondrial rhomboid protease, Presenilin-Associated Rhomboid Like (PARL), is a critical regulator of… read more here.
Keywords: protease; intramembrane; expression; purification human ... See more keywords
Expression and Purification of Dengue NS1 Protein in Sf9-Baculovirus System.
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DOI: 10.1007/978-1-0716-1879-0_4
Abstract: It is well known that glycosylations of Dengue NS1 protein are important for its structure, oligomerization, and immunogenicity. One of the major challenges in heterologous NS1 protein expression is the difference in glycosylation patterns amongst different organisms.… read more here.
Keywords: protein; ns1 protein; dengue ns1; expression purification ... See more keywords
Recombinant Expression and Purification of the Shigella Translocator IpaB.
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DOI: 10.1007/978-1-4939-6649-3_15
Abstract: Type III secretion systems (T3SS) are highly conserved virulence factors employed by a large number of pathogenic gram-negative bacteria. Like many T3SS translocators, recombinant expression of the hydrophobic Shigella protein IpaB requires the presence of… read more here.
Keywords: shigella; expression; ipab; expression purification ... See more keywords
Expression and Purification of N-Terminally His-Tagged Recombinant Type III Secretion Proteins.
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DOI: 10.1007/978-1-4939-6649-3_16
Abstract: The ability to express and purify recombinant needle proteins from the Type III Secretion System (T3SS) of many gram-negative bacteria has allowed us to develop novel experimental approaches, both in vitro and in vivo, to… read more here.
Keywords: iii secretion; expression purification; needle proteins; type iii ... See more keywords
Methods for Expression and Purification of Biologically Active Recombinant Progranulin.
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DOI: 10.1007/978-1-4939-8559-3_3
Abstract: Purifying progranulin may be useful in a variety of situations, for example, after it has been mutated or otherwise modified or when working with a species for which commercially produced progranulin is unavailable. A method… read more here.
Keywords: progranulin; methods expression; biologically active; purification ... See more keywords
Expression, Purification, and Crystallization of HSV-1 Glycoproteins for Structure Determination.
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DOI: 10.1007/978-1-4939-9814-2_23
Abstract: Herpes simplex viruses utilize glycoproteins displayed on the viral envelope to perform a variety of functions in the viral infectious cycle. Structural and functional studies of these viral glycoproteins can benefit from biochemical, biophysical, and structural analysis of… read more here.
Keywords: purification crystallization; hsv; expression purification;
ATP Synthase: Expression, Purification, and Function.
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DOI: 10.1007/978-1-4939-9869-2_5
Abstract: ATP synthase is an essential enzyme found in all known forms of life, generating the majority of cellular energy via a rotary catalytic mechanism. Here, we describe the in-depth methods for expression, purification, and functional… read more here.
Keywords: purification function; atp synthase; expression purification; synthase expression ... See more keywords
Expression and purification of recombinant extracellular sulfatase HSulf-2 allows deciphering of enzyme sub-domain coordinated role for the binding and 6-O-desulfation of heparan sulfate
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DOI: 10.1007/s00018-019-03027-2
Abstract: Through their ability to edit 6-O-sulfation pattern of Heparan sulfate (HS) polysaccharides, Sulf extracellular endosulfatases have emerged as critical regulators of many biological processes, including tumor progression. However, study of Sulfs remains extremely intricate and… read more here.
Keywords: expression purification; recombinant; domain; heparan sulfate ... See more keywords
Expression, Purification, and Evaluation of Anti-IL-2Rα Antibody Secreted by Leishmania tarentolae
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DOI: 10.1007/s10989-020-10088-6
Abstract: Producing a functional anti-IL-2R α antibody in Leishmania tarentolae , a trypanosomatid protozoan non-pathogenic to human, is a cost-effective and safe alternative for the production of therapeutic recombinant proteins. Expression cassettes encoding heavy and light… read more here.
Keywords: leishmania tarentolae; anti antibody; purification evaluation; antibody ... See more keywords
Heterologous expression, purification, and refolding of SRY protein: role of l-arginine as analyzed by simulation and practical study
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DOI: 10.1007/s11033-020-05667-1
Abstract: Escherichia coli is a widely-used cell factory for recombinant protein production, nevertheless, high amount of produced protein is seen in aggregated form. The purpose of this study was to improve the solubility of recombinant bovine… read more here.
Keywords: purification refolding; study; arginine; refolding sry ... See more keywords