Engineering of the CMV promoter for controlled expression of recombinant genes in HEK293 cells
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202200062
Abstract: Expression of recombinant genes in HEK293 cells is frequently utilized for production of recombinant proteins and viral vectors. These systems frequently employ the cytomegalovirus (CMV) promoter to drive recombinant gene transcription. However, the mechanistic basis… read more here.
Keywords: cmv; expression recombinant; recombinant genes; hek293 cells ... See more keywords
A Generic Protocol for Intracellular Expression of Recombinant Proteins in Bacillus subtilis.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6887-9_21
Abstract: Bacillus subtilis (B. subtilis) is a potential and attractive host for the production of recombinant proteins. Different expression systems for B. subtilis have been developed recently, and various target proteins have been recombinantly synthesized and… read more here.
Keywords: expression recombinant; generic protocol; expression; recombinant proteins ... See more keywords
Functional expression of recombinant hybrid enzymes composed of bacterial and insect's chitinase domains in E. coli.
Sign Up to like & getrecommendations! Published in 2020 at "Enzyme and microbial technology"
DOI: 10.1016/j.enzmictec.2019.109492
Abstract: To elucidate the functional alteration of the recombinant hybrid chitinases composed of bacterial and insect's domains, we cloned the constitutional domains from chitinase-encoding cDNAs of a bacterial species, Bacillus thuringiensis (BtChi) and a lepidopteran insect… read more here.
Keywords: composed bacterial; recombinant hybrid; expression recombinant; recombinant ... See more keywords
Application of chromosomal gene insertion into Escherichia coli for expression of recombinant proteins.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of bioscience and bioengineering"
DOI: 10.1016/j.jbiosc.2018.02.016
Abstract: Escherichia coli is the most popular organism used for producing recombinant proteins. However, the expression of recombinant proteins in E. coli sometimes results in the aggregation of proteins as an inclusion body in host cells. In… read more here.
Keywords: escherichia coli; insertion; expression recombinant; expression ... See more keywords
Efficient Transient Expression of Recombinant Proteins Using DNA Viral Vectors in Freshwater Microalgal Species
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2021.650820
Abstract: The increase in the world population, the advent of new infections and health issues, and the scarcity of natural biological products have spotlighted the importance of recombinant protein technology and its large-scale production in a… read more here.
Keywords: microalgal species; expression recombinant; recombinant; freshwater microalgal ... See more keywords