Enhanced cell density cultivation and rapid expression-screening of recombinant Pichia pastoris clones in microscale
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DOI: 10.1038/s41598-020-63995-5
Abstract: Cultivation of yeast Pichia pastoris in the microtiter plate, for optimisation of culture conditions, and expression screening of transformants has gained significance in recent years. However, in the microtiter plate, it has been challenging to… read more here.
Keywords: expression screening; cell density; cultivation; pichia pastoris ... See more keywords
HR-Bac, a toolbox based on homologous recombination for expression, screening and production of multiprotein complexes using the baculovirus expression system
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DOI: 10.1038/s41598-021-04715-5
Abstract: The Baculovirus/insect cell expression system is a powerful technology for reconstitution of eukaryotic macromolecular assemblies. Most multigene expression platforms rely on Tn7-mediated transposition for transferring the expression cassette into the baculoviral genome. This allows a… read more here.
Keywords: expression screening; expression; expression system; baculovirus ... See more keywords