Expression and Purification of SH2 Domains Using Baculovirus Expression System.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6762-9_11
Abstract: Recombinant proteins expressed in bacteria are sometimes insoluble, aggregated, and incorrectly folded. For those Src homology 2 (SH2) domains that are insoluble in bacteria, baculovirus-insect cell expression systems can be an alternative to produce soluble… read more here.
Keywords: sh2 domains; domains using; baculovirus expression; expression system ... See more keywords
Optimization of a 2A self-cleaving peptide-based multigene expression system for efficient expression of upstream and downstream genes in silkworm
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Genetics and Genomics"
DOI: 10.1007/s00438-019-01534-2
Abstract: The multigene expression system is highly attractive to co-express multiple genes or multi-subunit complex-based genes for their functional studies, and in gene therapy and visual tracking of expressed proteins. However, the current multiple gene co-expression… read more here.
Keywords: efficient expression; expression system; expression; gene ... See more keywords
Conditional constitutive expression system of a drug protein in vivo by positive feedback loop using an inducer-independent artificial transcription factor.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2017.12.133
Abstract: Bacterial-mediated drug delivery is a potential and promising strategy for the specific treatment of cancer with therapeutic molecules, especially with genetically encoded proteins. These proteins must be tightly regulated due to cytotoxicity and thus are… read more here.
Keywords: protein; expression system; expression; conditional constitutive ... See more keywords
A novel expression system for lytic polysaccharide monooxygenases.
Sign Up to like & getrecommendations! Published in 2017 at "Carbohydrate research"
DOI: 10.1016/j.carres.2017.02.003
Abstract: Lytic polysaccharide monooxygenases (LPMOs) are key enzymatic players of lignocellulosic biomass degradation processes. As such, they have been introduced in cellulolytic cocktails for more efficient and less expensive lignocellulose saccharification. The recombinant production of LPMOs… read more here.
Keywords: system; expression system; expression; polysaccharide monooxygenases ... See more keywords
A flavivirus-inducible gene expression system that modulates broad-spectrum antiviral activity against dengue and Zika viruses.
Sign Up to like & getrecommendations! Published in 2022 at "Insect biochemistry and molecular biology"
DOI: 10.1016/j.ibmb.2022.103723
Abstract: Incidence of dengue virus (DENV) and Zika virus (ZIKV), two mosquito-borne flaviviruses, is increasing in large parts of the world. Vaccination and medication for these diseases are unsatisfactory. Here, we developed a novel antiviral approach,… read more here.
Keywords: system; expression system; virus; gene expression ... See more keywords
Advanced multigene expression system for Nannochloropsis salina using 2A self-cleaving peptides.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2018.04.017
Abstract: Even though there has been much interest in genetic engineering of microalgae, its progress has been slow due to the difficulty and limitation of available techniques. Currently, genetic modification in most microalgal strains is confined… read more here.
Keywords: expression; expression system; multigene expression; nannochloropsis salina ... See more keywords
A co-expression system to shift the equilibrium of transamination reactions toward the synthesis of enantiomerically pure amines
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Catalysis"
DOI: 10.1016/j.mcat.2019.04.006
Abstract: Abstract The synthesis of chiral amines as important pharmaceutical and fine chemical building blocks can be carried out with amine transaminases. The reaction equilibrium is often unfavorable. There are several methods to shift the equilibrium… read more here.
Keywords: expression system; synthesis; shift equilibrium; dehydrogenase ... See more keywords
Proteins from Thermophilic Thermus thermophilus Often Do Not Fold Correctly in a Mesophilic Expression System Such as Escherichia coli
Sign Up to like & getrecommendations! Published in 2022 at "ACS Omega"
DOI: 10.1021/acsomega.2c04786
Abstract: Majority of protein structure studies use Escherichia coli (E. coli) and other model organisms as expression systems for other species’ genes. However, protein folding depends on cellular environment factors, such as chaperone proteins, cytoplasmic pH,… read more here.
Keywords: escherichia coli; thermus thermophilus; expression system; expression ... See more keywords
Establishing a Cell-Free Vibrio natriegens Expression System.
Sign Up to like & getrecommendations! Published in 2018 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.8b00222
Abstract: The fast growing bacterium Vibrio natriegens is an emerging microbial host for biotechnology. Harnessing its productive cellular components may offer a compelling platform for rapid protein production and prototyping of metabolic pathways or genetic circuits.… read more here.
Keywords: expression system; vibrio natriegens; biology; cell free ... See more keywords
A novel regulation mechanism of the T7 RNA polymerase based expression system improves overproduction and folding of membrane proteins
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-26668-y
Abstract: Membrane protein (MP) overproduction is one of the major bottlenecks in structural genomics and biotechnology. Despite the emergence of eukaryotic expression systems, bacteria remain a cost effective and powerful tool for protein production. The T7… read more here.
Keywords: novel regulation; rna polymerase; expression system; expression ... See more keywords
Antimicrobial genes from Allium sativum and Pinellia ternata revealed by a Bacillus subtilis expression system
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-32852-x
Abstract: Antimicrobial genes are found in all classes of life. To efficiently isolate these genes, we used Bacillus subtilis and Escherichia coli as target indicator bacteria and transformed them with cDNA libraries. Among thousands of expressed… read more here.
Keywords: bacillus subtilis; expression system; antimicrobial genes; subtilis expression ... See more keywords