Yeast Expression Systems: Overview and Recent Advances
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Biotechnology"
DOI: 10.1007/s12033-019-00164-8
Abstract: Yeasts are outstanding hosts for the production of functional recombinant proteins with industrial or medical applications. Great attention has been emerged on yeast due to the inherent advantages and new developments in this host cell.… read more here.
Keywords: overview recent; systems overview; yeast expression; expression ... See more keywords
Exploring three different expression systems for recombinant expression of globins: Escherichia coli, Pichia pastoris and Spodoptera frugiperda.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2017.11.027
Abstract: Globins are among the best investigated proteins in biological and medical sciences and represent a prime tool for the study of the evolution of genes and the structure-function relationship of proteins. Here, we explore the… read more here.
Keywords: different expression; expression globins; expression; expression systems ... See more keywords
Promoter regulation and genetic engineering strategies for enhanced cellulase expression in Trichoderma reesei.
Sign Up to like & getrecommendations! Published in 2022 at "Microbiological research"
DOI: 10.1016/j.micres.2022.127011
Abstract: BACKGROUND Trichoderma reesei has extraordinary potential for high-level protein production at large scales, and it need to be further explored through genetic engineering tools to obtain a thorough understanding of its cellular physiology. Understanding the… read more here.
Keywords: genetic engineering; expression; expression systems; trichoderma reesei ... See more keywords
Optimization of Heavy Metal Sensors Based on Transcription Factors and Cell-Free Expression Systems.
Sign Up to like & getrecommendations! Published in 2021 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.1c00331
Abstract: Many bacterial mechanisms for highly specific and sensitive detection of heavy metals and other hazards have been reengineered to serve as sensors. In some cases, these sensors have been implemented in cell-free expression systems, enabling… read more here.
Keywords: free expression; sensors based; cell free; expression systems ... See more keywords
Engineering dual-glycan responsive expression systems for tunable production of heterologous proteins in Bacteroides thetaiotaomicron
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-53726-w
Abstract: Genetically engineering intestinal bacteria, such as Bacteroides thetaiotaomicron (B. theta), holds potential for creating new classes of biological devices, such as diagnostics or therapeutic delivery systems. Here, we have developed a series of B. theta… read more here.
Keywords: engineering; glycan responsive; bacteroides thetaiotaomicron; dual glycan ... See more keywords
Establishment of transient gene expression systems in protoplasts from Liriodendron hybrid mesophyll cells
Sign Up to like & getrecommendations! Published in 2017 at "PLoS ONE"
DOI: 10.1371/journal.pone.0172475
Abstract: Liriodendron is a genus of the magnolia family comprised of two flowering tree species that produce hardwoods of great ecological and economic value. However, only a limited amount of genetic research has been performed on… read more here.
Keywords: liriodendron hybrid; expression systems; protoplasts liriodendron; gene ... See more keywords
Effective Use of Linear DNA in Cell-Free Expression Systems
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2021.715328
Abstract: Cell-free expression systems (CFEs) are cutting-edge research tools used in the investigation of biological phenomena and the engineering of novel biotechnologies. While CFEs have many benefits over in vivo protein synthesis, one particularly significant advantage… read more here.
Keywords: free expression; dna; cell free; expression ... See more keywords