CRISPR/Cas9-mediated knock-in strategy at the Rosa26 locus in cattle fetal fibroblasts
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DOI: 10.1371/journal.pone.0276811
Abstract: The genetic modification of cattle has many agricultural and biomedical applications. However, random integration often leads to the unstable or differentially expression of the exogenous genes, which limit the application and development of transgenic technologies.… read more here.
Keywords: crispr cas9; expression units; knock; different expression ... See more keywords