Multiple gene expression in plants using MIDAS‐P, a versatile type II restriction‐based modular expression vector
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28073
Abstract: MIDAS‐P is a plant expression vector with blue/white screening for iterative cloning of multiple, tandemly arranged transcription units (TUs). We have used the MIDAS‐P system to investigate the expression of up to five genes encoding… read more here.
Keywords: expression vector; multiple gene; anti hiv; expression ... See more keywords
In Silico Gene Analysis and Oligonucleotide Design for the Construction of Expression Vectors.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1048-0_20
Abstract: Recombinant expression of a gene of interest is a convenient method to study the function of the encoded protein. To introduce a gene into an expression vector, it is necessary to analyze the structure of… read more here.
Keywords: expression vector; expression; gene analysis; gene ... See more keywords
A co-expression vector for baculovirus-mediated protein expression in mammalian cells.
Sign Up to like & getrecommendations! Published in 2022 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2022.01.056
Abstract: BacMam system utilizes baculovirus to deliver exogenous genes into mammalian cells and is extensively used for recombinant production of eukaryotic proteins. Here, we described the development of a BacMam vector (pBMCL1), which allows convenient tracing… read more here.
Keywords: vector baculovirus; expression vector; expression; mammalian cells ... See more keywords
Construction and expression of a prokaryotic expression vector for the goat sry gene
Sign Up to like & getrecommendations! Published in 2019 at "Indian Journal of Animal Research"
DOI: 10.18805/ijar.b-963
Abstract: Goats are economically important animals in the world, and their sex is an important factor in their economic efficiency. Reconstruction of a goat SRY gene expression vector can lay a foundation for studying the immunogenicity… read more here.
Keywords: expression; sry; expression vector; gene ... See more keywords
A Bacterial Expression Vector Archive (BEVA) for Flexible Modular Assembly of Golden Gate-Compatible Vectors
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2018.03345
Abstract: We present a Bacterial Expression Vector Archive (BEVA) for the modular assembly of bacterial vectors compatible with both traditional and Golden Gate cloning, utilizing the Type IIS restriction enzyme Esp3I, and have demonstrated its use… read more here.
Keywords: vector; golden gate; expression vector; bacterial expression ... See more keywords
Construction of a chalcone reductase expression vector and transformation of soybean plants.
Sign Up to like & getrecommendations! Published in 2017 at "Molecular medicine reports"
DOI: 10.3892/mmr.2017.7382
Abstract: The present study aimed to clone the soybean chalcone reductase 3 (CHR3) and create a recombinant expression vector pCAMBIA3300‑CHR3 containing Bar resistance gene as a selection marker, and then obtain transgenic soybean plants using Agrobacterium… read more here.
Keywords: expression vector; soybean; chr3; expression ... See more keywords