Mammalian expression vectors for metabolic biotinylation tandem affinity tagging by co-expression in cis of a mammalian codon-optimized BirA biotin ligase
Sign Up to like & getrecommendations! Published in 2018 at "BMC Research Notes"
DOI: 10.1186/s13104-018-3500-9
Abstract: AbstractΟbjectiveTo construct mammalian expression vectors for the N- or C-terminal tagging of proteins with a tandem affinity tag comprised of the biotinylatable Avi tag and of a triple FLAG tag.ResultsWe constructed and tested by transient… read more here.
Keywords: tandem affinity; expression; tag; expression vectors ... See more keywords
Development of a set of novel binary expression vectors for plant gene function analysis and genetic transformation
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2022.1104905
Abstract: With the advent of multiple omics and Genome-Wide Association Studies (GWAS) technology, genome-scale functional analysis of candidate genes is to be conducted in diverse plant species. Construction of plant binary expression vectors is the prerequisite… read more here.
Keywords: analysis; expression vectors; plant; gene ... See more keywords
Successful Rescue of Synthetic AcMNPV with a ~17 kb Deletion in the C1 Region of the Genome
Sign Up to like & getrecommendations! Published in 2022 at "Viruses"
DOI: 10.3390/v14122780
Abstract: Baculoviruses have been widely used as expression vectors. However, numerous genes in the baculoviral genome are non-essential for cellular infection and protein expression, making the optimisation of baculovirus expression vectors possible. We used a synthetic… read more here.
Keywords: baculovirus expression; region; genome; expression vectors ... See more keywords