Using overlapâextension PCR technique to fusing genes for constructing recombinant plasmids
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Basic Microbiology"
DOI: 10.1002/jobm.201700455
Abstract: Overlapâextension PCR is a method for splice of gene segments to produce focused fragments for constructing recombinant plasmid, but its complexity limits its application. To simplify the protocol and to improve the effectiveness, we employed… read more here.
Keywords: constructing recombinant; overlap extension; using overlap; extension pcr ... See more keywords
Gibson assembly interposition improves amplification efficiency of long DNA and multifragment overlap extension PCR.
Sign Up to like & getrecommendations! Published in 2023 at "BioTechniques"
DOI: 10.2144/btn-2023-0012
Abstract: For difficult overlap extension PCR, a Gibson assembly process was inserted between the two PCR rounds to facilitate the formation of complete gene templates at a moderate temperature. That is, after amplifying each DNA fragment,… read more here.
Keywords: overlap extension; gibson assembly; extension pcr; pcr ... See more keywords
A Python script to design primers for overlap extension PCR to ligate two DNA fragments
Sign Up to like & getrecommendations! Published in 2022 at "PeerJ"
DOI: 10.7717/peerj.14283
Abstract: Ligating two or more DNA fragments is a regular operation for the subcloning and the engineering of vectors. The overlap extension PCR serves as a straightforward method to solve this issue. However, it takes a… read more here.
Keywords: overlap extension; python script; dna fragments; design ... See more keywords