Novel standardized method for extracellular flux analysis of oxidative and glycolytic metabolism in peripheral blood mononuclear cells
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-81217-4
Abstract: Analyzing metabolism of peripheral blood mononuclear cells (PBMCs) provides key opportunities to study the pathophysiology of several diseases, such as type 2 diabetes, obesity and cancer. Extracellular flux (XF) assays provide dynamic metabolic analysis of… read more here.
Keywords: pbmcs; flux analysis; analysis; method ... See more keywords
Extracellular flux analysis in intact cardiac tissue slices - a novel tool to investigate cardiac substrate metabolism in mouse myocardium.
Sign Up to like & getrecommendations! Published in 2023 at "Acta physiologica"
DOI: 10.1111/apha.14004
Abstract: AIM Cardiac pathologies are accompanied by alterations in substrate metabolism, and extracellular flux analysis is a standard tool to investigate metabolic disturbances, especially in immortalized cell lines. However, preparations of primary cells, such as adult… read more here.
Keywords: metabolism; tissue slices; extracellular flux; flux analysis ... See more keywords
Activation of retinal glial (Müller) cells by extracellular ATP induces pronounced increases in extracellular H+ flux
Sign Up to like & getrecommendations! Published in 2018 at "PLoS ONE"
DOI: 10.1371/journal.pone.0190893
Abstract: Small alterations in extracellular acidity are potentially important modulators of neuronal signaling within the vertebrate retina. Here we report a novel extracellular acidification mechanism mediated by glial cells in the retina. Using self-referencing H+-selective microelectrodes… read more here.
Keywords: atp; extracellular atp; ller cells; activation ... See more keywords
Real-time assessment of neutrophil metabolism and oxidative burst using extracellular flux analysis
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2023.1083072
Abstract: Neutrophil responses are critical during inflammatory and infective events, and neutrophil dysregulation has been associated with poor patient outcomes. Immunometabolism is a rapidly growing field that has provided insights into cellular functions in health and… read more here.
Keywords: metabolism; extracellular flux; oxidative burst; analysis ... See more keywords