Extracellular Secretion and Simple Purification of Bacterial Collagen from Escherichia coli.
Sign Up to like & getrecommendations! Published in 2022 at "Biomacromolecules"
DOI: 10.1021/acs.biomac.1c01191
Abstract: Because of structural similarities with type-I animal collagen, recombinant bacterial collagen-like proteins have been progressively used as a source of collagen for biomaterial applications. However, the intracellular expression combined with current costly and time-consuming chromatography… read more here.
Keywords: purification; collagen; bacterial collagen; extracellular secretion ... See more keywords
A two-stage temperature control strategy enhances extracellular secretion of recombinant α-cyclodextrin glucosyltransferase in Escherichia coli
Sign Up to like & getrecommendations! Published in 2017 at "AMB Express"
DOI: 10.1186/s13568-017-0465-3
Abstract: The effects of temperature on extracellular secretion of the α-cyclodextrin glucosyltransferase (α-CGTase) from Paenibacillus macerans JFB05-01 by Escherichia coli were investigated. When protein expression was induced at constant temperature, the greatest amount of extracellular recombinant… read more here.
Keywords: temperature; cyclodextrin glucosyltransferase; extracellular secretion; recombinant ... See more keywords