RNA-extraction-free nano-amplified colorimetric test for point-of-care clinical diagnosis of COVID-19.
Sign Up to like & getrecommendations! Published in 2021 at "Nature protocols"
DOI: 10.1038/s41596-021-00546-w
Abstract: The global pandemic of coronavirus disease 2019 (COVID-19) highlights the shortcomings of the current testing paradigm for viral disease diagnostics. Here, we report a stepwise protocol for an RNA-extraction-free nano-amplified colorimetric test for rapid and… read more here.
Keywords: rna extraction; nano amplified; free nano; extraction free ... See more keywords
149. Extraction-free RT-PCR to Detect SARS-CoV-2 Variants of Concern
Sign Up to like & getrecommendations! Published in 2021 at "Open Forum Infectious Diseases"
DOI: 10.1093/ofid/ofab466.149
Abstract: Abstract Background SARS-CoV-2 variants of concern (VOC) have challenged real-time reverse transcriptase polymerase chain reaction (RT-PCR) methods for the diagnosis of COVID-19. Methods The CDC 2019-Novel Coronavirus real-time RT-PCR panel was modified to create a… read more here.
Keywords: sars cov; pcr; cov variants; mut ... See more keywords
Extraction-Free Methods for the Detection of SARS-CoV-2 by Reverse Transcription-PCR: a Comparison with the Cepheid Xpert Xpress SARS-CoV-2 Assay across Two Medical Centers
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Clinical Microbiology"
DOI: 10.1128/jcm.02643-20
Abstract: RT-PCR is the gold-standard for the diagnosis of SARS-CoV-2 infection, however, testing has been complicated by supply shortages and long turn-around times.…. read more here.
Keywords: cov reverse; detection sars; extraction free; free methods ... See more keywords
Strategies That Facilitate Extraction-Free SARS-CoV-2 Nucleic Acid Amplification Tests
Sign Up to like & getrecommendations! Published in 2022 at "Viruses"
DOI: 10.3390/v14061311
Abstract: The COVID-19 pandemic has resulted in an unprecedented global demand for in vitro diagnostic reagents. Supply shortages and hoarding have impacted testing capacity which has led to inefficient COVID-19 case identification and transmission control, predominantly… read more here.
Keywords: cov nucleic; nucleic acid; extraction; extraction free ... See more keywords