Bovine milk microbiota: Evaluation of different DNA extraction protocols for challenging samples
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DOI: 10.1002/mbo3.1275
Abstract: The use of an adequate protocol that accurately extracts microbial DNA from bovine milk samples is of importance for downstream analysis such as 16S ribosomal RNA gene sequencing. Although sequencing platforms such as Illumina are… read more here.
Keywords: dna; bovine milk; extraction; extraction protocols ... See more keywords
Improved nucleic acid extraction protocols for Ganoderma boninense, G. miniatocinctum and G. tornatum
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology Letters"
DOI: 10.1007/s10529-018-2603-7
Abstract: The first and most crucial step of all molecular techniques is to isolate high quality and intact nucleic acids. However, DNA and RNA isolation from fungal samples are usually difficult due to the cell walls… read more here.
Keywords: boninense miniatocinctum; extraction; tornatum; extraction protocols ... See more keywords
Assessment of fecal DNA extraction protocols for metagenomic studies
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DOI: 10.1093/gigascience/giaa071
Abstract: Abstract Background Shotgun metagenomic sequencing has improved our understanding of the human gut microbiota. Various DNA extraction methods have been compared to find protocols that robustly and most accurately reflect the original microbial community structures.… read more here.
Keywords: extraction; dna; extraction protocols; protocol ... See more keywords
Analysis of Intracellular Metabolites from Microorganisms: Quenching and Extraction Protocols
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DOI: 10.3390/metabo7040053
Abstract: Sample preparation is one of the most important steps in metabolome analysis. The challenges of determining microbial metabolome have been well discussed within the research community and many improvements have already been achieved in last… read more here.
Keywords: extraction; analysis; extraction protocols; analysis intracellular ... See more keywords