Predictive mechanistic model for separation of monoclonal antibody, fab fragment, and aggregate species on multimodal chromatography
Sign Up to like & getrecommendations! Published in 2025 at "Biotechnology Progress"
DOI: 10.1002/btpr.70022
Abstract: The specific selectivities offered by multimodal ligands drive the increased application of multimodal chromatography in the purification of complex new “multispecific” antibodies, which requires improved understanding of the protein‐multimodal ligand interaction mechanism. In the present… read more here.
Keywords: monoclonal antibody; fab fragment; mab1 fab; model ... See more keywords
Structure of human DPEP3 in complex with the SC-003 antibody Fab fragment reveals basis for lack of dipeptidase activity.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of structural biology"
DOI: 10.1016/j.jsb.2020.107512
Abstract: Dipeptidase 3 (DPEP3) is one of three glycosylphosphatidylinositol-anchored metallopeptidases potentially involved in the hydrolytic metabolism of dipeptides. While its exact biological function is not clear, DPEP3 expression is normally limited to testis , but can… read more here.
Keywords: fab fragment; dipeptidase; dpep3; antibody ... See more keywords
Fab Fragment of Immunoglobulin Y Modulates NF-κB and MAPK Signaling through TLR4 and αVβ3 Integrin and Inhibits the Inflammatory Effect on R264.7 Macrophages.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.1c03330
Abstract: High-purity Fab fragment and immunoglobulin Y (IgY) were prepared to evaluate their anti-inflammatory activity in the lipopolysaccharide (LPS)-induced Raw 264.7 macrophage system. Compared with IgY, the Fab fragment possessed a greater potency in inhibiting the… read more here.
Keywords: fab fragment; fragment immunoglobulin; inhibition; fragment ... See more keywords
Enhanced Thermal Stability and Reduced Aggregation in an Antibody Fab Fragment at Elevated Concentrations
Sign Up to like & getrecommendations! Published in 2023 at "Molecular Pharmaceutics"
DOI: 10.1021/acs.molpharmaceut.3c00081
Abstract: The aggregation of protein therapeutics such as antibodies remains a major challenge in the biopharmaceutical industry. The present study aimed to characterize the impact of the protein concentration on the mechanisms and potential pathways for… read more here.
Keywords: aggregation; antibody fab; concentration; rate ... See more keywords
Optimization and Validation of the Cationization Method for the Fab’ Fragment of Antibody Rituximab
Sign Up to like & getrecommendations! Published in 2025 at "ACS Omega"
DOI: 10.1021/acsomega.4c07727
Abstract: This study aimed to optimize and validate a cationization method for the Fab’ fragment of antibody rituximab, following the guidelines set by the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use… read more here.
Keywords: method; fab fragment; antibody rituximab; cationization method ... See more keywords
The Fab fragment of anti-IgE Cε2 domain prevents allergic reactions through interacting with IgE-FcεRIα complex on rat mast cells
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-32200-z
Abstract: Immunoglobulin E (IgE) plays a central role in the pathogenesis of Type I hypersensitivity through interaction with a high-affinity receptor (FcεRIα). For therapeutic applications, substantial attention has been focused recently on the blockade of the… read more here.
Keywords: fab fragment; ige; fab 6hd5; ige complex ... See more keywords
Structural insight to elucidate the binding specificity of the anti-cortisol Fab fragment with glucocorticoids.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of structural biology"
DOI: 10.2139/ssrn.4367514
Abstract: Cortisol is a steroid hormone that is produced by the adrenal gland. It is a primary stress hormone that increases glucose levels in the blood stream. High concentrations of cortisol in the body can be… read more here.
Keywords: cortisol fab; binding specificity; anti cortisol; cortisol ... See more keywords