Dissecting Molecular Phenotypes Through FACS-Based Pooled CRISPR Screens.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/7651_2021_457
Abstract: Pooled CRISPR screens are emerging as a powerful tool to dissect regulatory networks, by assessing how a protein responds to genetic perturbations in a highly multiplexed manner. A large number of genes are perturbed in… read more here.
Keywords: molecular phenotypes; crispr screens; facs based; dissecting molecular ... See more keywords
A FACS-Based Genome-wide CRISPR Screen Reveals a Requirement for COPI in Chlamydia trachomatis Invasion
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DOI: 10.1016/j.isci.2018.12.011
Abstract: The invasion of Chlamydia trachomatis, an obligate intracellular bacterium, into epithelial cells is driven by a complex interplay of host and bacterial factors. To comprehensively define the host genes required for pathogen invasion, we undertook… read more here.
Keywords: invasion; chlamydia trachomatis; facs based; trachomatis ... See more keywords
An optimized dissociation protocol for FACS-based isolation of rare cell types from Caenorhabditis elegans L1 larvae
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DOI: 10.1016/j.mex.2020.100922
Abstract: Single-cell isolation and transcriptomic analysis of a specific cell type or tissue offers the possibility of studying cell function and heterogeneity in time-dependent processes with remarkable resolution. The reduced tissue complexity and highly stereotyped development… read more here.
Keywords: elegans larvae; caenorhabditis elegans; cell types; facs based ... See more keywords
A FACS-based screening strategy to assess sequence-specific RNA-binding of Pumilio protein variants in E. coli
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DOI: 10.1515/hsz-2016-0214
Abstract: Abstract Sequence-specific and programmable binding of proteins to RNA bears the potential to detect and manipulate target RNAs. Applications include analysis of subcellular RNA localization or post-transcriptional regulation but require sequence-specificity to be readily adjustable… read more here.
Keywords: sequence specific; pumilio; rna; facs based ... See more keywords