Multicenter performance evaluation and reference range determination of a new one‐stage factor VIII assay
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.24294
Abstract: We conducted a multicenter evaluation of a new one‐stage factor VIII (FVIII) assay (Roche Diagnostics), intended for the quantitative assessment of FVIII activity. We evaluated the analytical performance of the FVIII assay on the cobas… read more here.
Keywords: evaluation; one stage; multicenter; factor viii ... See more keywords
Chromogenic Factor VIII Assays for Improved Diagnosis of Hemophilia A.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7196-1_21
Abstract: Hemophilia A is an inherited bleeding disorder caused by a reduced level of factor VIII coagulant activity (FVIII:C) in blood. Bleeding episodes may occur spontaneously in the severe form of hemophilia or after trauma in… read more here.
Keywords: hemophilia; factor viii; chromogenic factor; diagnosis ... See more keywords
Quantification of Coagulation Factor VIII by Selective Reaction Monitoring.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7312-5_22
Abstract: Coagulation factor VIII (FVIII) is an important glycoprotein involved in the extrinsic coagulation cascade. Mutations in FVIII gene results in hemophilia A, a recessive coagulation disorder that is clinically managed by administration of purified FVIII… read more here.
Keywords: coagulation; reaction monitoring; factor viii; coagulation factor ... See more keywords
Subcutaneous administration of Lyso‐phosphatidylserine nanoparticles induces immunological tolerance towards Factor VIII in a Hemophilia A mouse model
Sign Up to like & getrecommendations! Published in 2018 at "International Journal of Pharmaceutics"
DOI: 10.1016/j.ijpharm.2018.07.018
Abstract: ABSTRACT A major complication with enzyme replacement therapy of Factor VIII (FVIII) in Hemophilia A (HA) is the development of anti‐drug antibodies. Recently, we have shown that FVIII administration in the presence of heterogeneous phosphatidylserine… read more here.
Keywords: administration; tolerance towards; administration lyso; tolerance ... See more keywords
Factor VIII/protein C ratio independently predicts liver-related events but does not indicate a hypercoagulable state in ACLD.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of hepatology"
DOI: 10.1016/j.jhep.2021.12.038
Abstract: BACKGROUND&AIMS It has been suggested that the ratio of procoagulant factor VIII to anticoagulant protein C (FVIII/PC) reflects the haemostatic equilibrium. Moreover, FVIII/PC predicted decompensation/death in a small study not accounting for portal hypertension severity.… read more here.
Keywords: hypercoagulable state; liver disease; fviii; factor viii ... See more keywords
Choice of factor VIII/IX regimen in adolescents and young adults with severe or moderately severe haemophilia. A French national observational study (ORTHem 15-25).
Sign Up to like & getrecommendations! Published in 2017 at "Thrombosis research"
DOI: 10.1016/j.thromres.2016.12.023
Abstract: INTRODUCTION The value and challenges of long-term prophylaxis (LTP) in adolescents and young adults need further characterisation. AIM To determine the proportions of adolescents and young adults with severe or moderately severe haemophilia in France… read more here.
Keywords: adolescents young; treatment; young adults; factor viii ... See more keywords
Effects of Product Handling Parameters on Particle Levels in a Commercial Factor VIII Product: Impacts and Mitigation.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of pharmaceutical sciences"
DOI: 10.1016/j.xphs.2018.08.022
Abstract: To reduce the risk of immunogenicity that may be caused by therapeutic protein products, it is important to properly characterize subvisible particles and to develop strategies to reduce the levels of particles delivered to patients.… read more here.
Keywords: particle levels; viii product; product; factor viii ... See more keywords
Polysialic acid-mediated activity measurement of polysialylated recombinant coagulation factor VIII.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of pharmaceutical sciences"
DOI: 10.1016/j.xphs.2019.10.019
Abstract: Measurement of modified biologic including coagulation factors with extended half-life obtained for example by polysialylation pose an analytical challenge especially if both biological activity and presence of modification have to be determined. Analytical methods applied… read more here.
Keywords: polysialic acid; acid mediated; factor viii; polysialylated recombinant ... See more keywords
Pre-clinical evaluation of an enhanced-function factor VIII variant for durable hemophilia A gene therapy in male mice
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-024-51296-8
Abstract: Durable factor VIII expression that normalizes hemostasis is an unrealized goal of hemophilia A adeno-associated virus-mediated gene therapy. Trials with initially normal factor VIII activity observed unexplained year-over-year declines in expression while others reported low-level,… read more here.
Keywords: expression; factor; factor viii; gene therapy ... See more keywords
Chacma baboon natural anticoagulants and factor VIII activities
Sign Up to like & getrecommendations! Published in 2025 at "Scientific Reports"
DOI: 10.1038/s41598-025-94503-2
Abstract: Non-human primates are useful models of human haemostasis, but distinctions do exist. These differences are essential to identify and quantify to better characterise the Chacma baboon’s coagulation system. The Chacma baboon’s factor VIII and natural… read more here.
Keywords: protein; chacma baboon; natural anticoagulants; factor viii ... See more keywords
Binding Promiscuity of Therapeutic Factor VIII
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DOI: 10.1055/a-2358-0853
Abstract: Abstract The binding promiscuity of proteins defines their ability to indiscriminately bind multiple unrelated molecules. Binding promiscuity is implicated, at least in part, in the off-target reactivity, nonspecific biodistribution, immunogenicity, and/or short half-life of potentially… read more here.
Keywords: promiscuity; factor viii; therapeutic factor; promiscuity therapeutic ... See more keywords