RecJ 5' Exonuclease Digestion of Oligonucleotide Failure Strands: A "Green" Method of Trityl-On Purification.
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DOI: 10.1021/acs.biochem.7b00010
Abstract: Methods of error filtration and correction post-gene assembly are a major bottleneck in the synthetic biology pipeline. Current oligonucleotide purification strategies, including polyacrylamide gel electrophoresis and high-performance liquid chromatography, are often expensive and labor-intensive, give… read more here.
Keywords: trityl purification; exonuclease; purification; failure strands ... See more keywords