Enhanced H2 production by deletion of the Tfx family DNA-binding protein in the hyperthermophilic archaeon Thermococcus onnurineus NA1
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Hydrogen Energy"
DOI: 10.1016/j.ijhydene.2021.08.075
Abstract: Abstract A gene encoding an archaeal transcription regulator (TON_1525) homologous to the Tfx family DNA-binding protein has been identified to affect gene expression of carbon monoxide metabolism in the hyperthermophilic archaeon Thermococcus onnurineus NA1. To… read more here.
Keywords: family dna; tfx family; dna binding; onnurineus na1 ... See more keywords
A Transition-State Perspective on Y-Family DNA Polymerase η Fidelity in Comparison with X-Family DNA Polymerases λ and β.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemistry"
DOI: 10.1021/acs.biochem.9b00087
Abstract: Deoxynucleotide misincorporation efficiencies can span a wide 104-fold range, from ∼10-2 to ∼10-6, depending principally on polymerase (pol) identity and DNA sequence context. We have addressed DNA pol fidelity mechanisms from a transition-state (TS) perspective… read more here.
Keywords: family dna; fidelity; dna; family ... See more keywords
TT(N)mGCCTC inhibits archaeal family B DNA polymerases
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-20127-4
Abstract: The proofreading activity of the archaeal family B DNA polymerases enables PCR with high fidelity. However, thermostable proofreading DNA polymerases occasionally failed to amplify target fragment that could be amplified by Taq DNA polymerase. We… read more here.
Keywords: family dna; dna; dna polymerases; archaeal family ... See more keywords
Diverse roles of RAD18 and Y-family DNA polymerases in tumorigenesis
Sign Up to like & getrecommendations! Published in 2018 at "Cell Cycle"
DOI: 10.1080/15384101.2018.1456296
Abstract: ABSTRACT Mutagenesis is a hallmark and enabling characteristic of cancer cells. The E3 ubiquitin ligase RAD18 and its downstream effectors, the ‘Y-family’ Trans-Lesion Synthesis (TLS) DNA polymerases, confer DNA damage tolerance at the expense of… read more here.
Keywords: family dna; dna; rad18 family; dna polymerases ... See more keywords
Family D DNA polymerase interacts with GINS to promote CMG-helicase in the archaeal replisome.
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic acids research"
DOI: 10.1093/nar/gkab799
Abstract: Genomic DNA replication requires replisome assembly. We show here the molecular mechanism by which CMG (GAN-MCM-GINS)-like helicase cooperates with the family D DNA polymerase (PolD) in Thermococcus kodakarensis. The archaeal GINS contains two Gins51 subunits,… read more here.
Keywords: family dna; dna; dna polymerase; helicase ... See more keywords
Reclassification of family A DNA polymerases reveals novel functional subfamilies and distinctive structural features
Sign Up to like & getrecommendations! Published in 2023 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkad242
Abstract: Abstract Family A DNA polymerases (PolAs) form an important and well-studied class of extant polymerases participating in DNA replication and repair. Nonetheless, despite the characterization of multiple subfamilies in independent, dedicated works, their comprehensive classification… read more here.
Keywords: polymerases reveals; dna; reclassification family; family dna ... See more keywords
A Well-Conserved Archaeal B-Family Polymerase Functions as an Extender in Translesion Synthesis
Sign Up to like & getrecommendations! Published in 2022 at "mBio"
DOI: 10.1128/mbio.02659-21
Abstract: In this work, we report that Sulfolobus islandicus Dpo2, a B-family DNA polymerase once predicted to be an inactive form, is a bona fide DNA polymerase functioning in translesion synthesis. S. islandicus Dpo2 is a… read more here.
Keywords: dna; translesion synthesis; dna polymerase; polymerase ... See more keywords
Translesion synthesis of apurinic/apyrimidic siteanalogues by Y-family DNA polymerase Dbh from Sulfolobus acidocaldarius
Sign Up to like & getrecommendations! Published in 2022 at "Acta Biochimica et Biophysica Sinica"
DOI: 10.3724/abbs.2022045
Abstract: Apurinic/apyrimidic (AP) sites are severe DNA damages and strongly block DNA extension by major DNA polymerases. Y-family DNA polymerases possess a strong ability to bypass AP sites and continue the DNA synthesis reaction, which is… read more here.
Keywords: dna; synthesis; family dna; tls ... See more keywords