Extending the detectable time window of fast protein dynamics using 1HN E-CPMG
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DOI: 10.1007/s10858-025-00470-1
Abstract: Recent advances in high power NMR relaxation dispersion experiments have significantly enhanced our ability to study fast µs timescale motions in proteins, which are crucial for understanding their biological functions. Here, we have extended the… read more here.
Keywords: time window; fast protein; window fast; protein dynamics ... See more keywords
Fluorescence Lifetime Multiplexing with Fluorogen-Activating FAST Protein Variants and Red-Shifted Arylidene–Imidazolone Derivative as Fluorogen
Sign Up to like & getrecommendations! Published in 2025 at "Biosensors"
DOI: 10.3390/bios15050274
Abstract: Fluorescence-lifetime imaging microscopy (FLIM) is a powerful technique for highly multiplexed imaging in live cells. In this work, we present a genetically encoded FLIM multiplexing platform based on a combination of fluorogen-activating protein FAST and… read more here.
Keywords: fluorescence lifetime; fluorogen; fast protein; arylidene imidazolone ... See more keywords