P173 The ART(EFACT) of multicolor flow cytometry. A case of mysterious CD3/CD19 double positive lymphocytes in the flow cytometry crossmatch (FCXM) assay
Sign Up to like & getrecommendations! Published in 2017 at "Human Immunology"
DOI: 10.1016/j.humimm.2017.06.233
Abstract: Aim Over the past 3 months we have been noticing an unusual population of CD3/CD19 double positive (DP) lymphocytes in some flowcytometry crossmatches (FCXM) performed in our HLA lab. Initially, the appearance of these unusual DP… read more here.
Keywords: flow cytometry; cd3 cd19; cd3; fcxm ... See more keywords
P119 Correlation between the strength of donor specific antibody (DSA) using single antigen bead assay (SAB) and the results of CDC- cytotoxicity and flow cytometric crossmatch
Sign Up to like & getrecommendations! Published in 2018 at "Human Immunology"
DOI: 10.1016/j.humimm.2018.07.176
Abstract: Aim In this study we correlated flow and CDC- crossmatch outcomes with results of Luminex-single antigen bead (SAB). Methods Between 2010–2016 HLA-antibody Sera were tested by screening and SAB class I & II (One lambda)… read more here.
Keywords: crossmatch; antibody; hla; correlation ... See more keywords
P137 Go pro(nase)! the importance of donor lymphocyte treatment with pronase in flow cytometric crossmatch testing
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DOI: 10.1016/j.humimm.2018.07.194
Abstract: Aim Flow cytometric crossmatch (FCXM) is performed to confirm donor/recipient histocompatibility. Previous multicenter study (Liwski et al. ASHI 2012) suggested that protocol differences contribute to FCXM result variability and that pronase treatment improves B cell… read more here.
Keywords: treatment; cytometric crossmatch; flow cytometric; donor ... See more keywords
Comparison of New OneLambda Flow DSA-XM With Conventional Flow Crossmatch
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DOI: 10.1097/01.tp.0000543530.79282.ca
Abstract: Background Flow crossmatching(FCXM) has improved detection of low level anti-HLA antibody. However, FCXM doesn't accurately discriminate anti-Class I vs. Class II antibody, may detect non-HLA antibodies and may increase false positives, particularly against B cell… read more here.
Keywords: positive negative; class; dsa; flow dsa ... See more keywords
Predictive Potential of Flow Cytometry Crossmatching in Deceased Donor Kidney Transplant Recipients Subjected to Peritransplant Desensitization
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DOI: 10.3389/fmed.2021.780636
Abstract: Recipient sensitization is a major risk factor of antibody-mediated rejection (ABMR) and inferior graft survival. The predictive effect of solid-phase human leukocyte antigen antibody testing and flow cytometry crossmatch (FCXM) in the era of peritransplant… read more here.
Keywords: flow cytometry; peritransplant desensitization; desensitization; fcxm ... See more keywords