Recommendations for Comparison of Productivity Between Fed‐Batch and Perfusion Processes
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DOI: 10.1002/biot.201700721
Abstract: Due to the growing interest in integrated continuous processing in the biopharmaceutical industry, productivity comparison of batch‐based and continuous processes is considered a challenge. Integrated continuous manufacturing of biopharmaceuticals requires scientists and engineers to collaborate… read more here.
Keywords: productivity; recommendations comparison; comparison productivity; productivity fed ... See more keywords
Online optimal experimental re‐design in robotic parallel fed‐batch cultivation facilities
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DOI: 10.1002/bit.26192
Abstract: We present an integrated framework for the online optimal experimental re‐design applied to parallel nonlinear dynamic processes that aims to precisely estimate the parameter set of macro kinetic growth models with minimal experimental effort. This… read more here.
Keywords: experimental design; cultivation; fed batch; optimal experimental ... See more keywords
Cell‐controlled hybrid perfusion fed‐batch CHO cell process provides significant productivity improvement over conventional fed‐batch cultures
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26259
Abstract: A simple method originally designed to control lactate accumulation in fed‐batch cultures of Chinese Hamster Ovary (CHO) cells has been modified and extended to allow cells in culture to control their own rate of perfusion… read more here.
Keywords: perfusion; batch cultures; fed batch; batch ... See more keywords
Metabolic phenotyping of CHO cells varying in cellular biomass accumulation and maintenance during fed‐batch culture
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DOI: 10.1002/bit.26485
Abstract: CHO cell lines capable of high‐level recombinant protein product biosynthesis during fed‐batch culture are still generally obtained by intensive empirical screening of transfected cells rather than knowledge‐guided cellular engineering. In this study, we investigate how… read more here.
Keywords: batch culture; fed batch; cho; cho cell ... See more keywords
Bioreactor productivity and media cost comparison for different intensified cell culture processes
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DOI: 10.1002/btpr.2415
Abstract: Process intensification in biomanufacturing has attracted a great deal of interest in recent years. Manufacturing platform improvements leading to higher cell density and bioreactor productivity have been pursued. Here we evaluated a variety of intensified… read more here.
Keywords: perfusion; productivity; fed batch; batch ... See more keywords
High solid fed‐batch butanol fermentation with simultaneous product recovery: Part II—process integration
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DOI: 10.1002/btpr.2643
Abstract: In these studies, liquid hot water (LHW) pretreated and enzymatically hydrolyzed Sweet Sorghum Bagasse (SSB) hydrolyzates were fermented in a fed‐batch reactor. As reported in the preceding paper, the culture was not able to ferment… read more here.
Keywords: recovery; fermentation; process; fed batch ... See more keywords
Development of a scale‐up strategy for Chinese hamster ovary cell culture processes using the kLa ratio as a direct indicator of gas stripping conditions
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DOI: 10.1002/btpr.3000
Abstract: Herein, we described a scale‐up strategy focused on the dissolved carbon dioxide concentration (dCO2) during fed‐batch cultivation of Chinese hamster ovary cells. A fed‐batch culture process for a 2000‐L scale stainless steel (SS) bioreactor was… read more here.
Keywords: kla ratio; bioreactor; fed batch; dco2 ... See more keywords
Antibody glycation during a CHO fed-batch process following a constrained second order reaction.
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DOI: 10.1002/btpr.3261
Abstract: Glycation on lysine side chains of recombinant monoclonal antibodies (mAb) is a well-known phenomenon in manufacturing processes of biopharmaceuticals that potentially alter the efficacy of the therapeutic protein. In the present study, we report kinetic… read more here.
Keywords: cho fed; fed batch; order; reaction ... See more keywords
Optimized pulse‐feeding fed‐batch fermentation for enhanced lignin to polyhydroxyalkanoate transformation
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DOI: 10.1002/btpr.3302
Abstract: With an anticipated growth of Bio‐Circular‐Green economy, the amount of lignin generated as by‐product from biorefineries is increasing. Hence, lignin valorising strategies become a very interesting option to improve economic viability of the biorefineries. This… read more here.
Keywords: lignin; fed batch; pulse feeding; pha ... See more keywords
Orbitally Shaken Single-Use Bioreactor for Animal Cell Cultivation: Fed-Batch and Perfusion Mode.
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DOI: 10.1007/978-1-0716-0191-4_7
Abstract: Increasing the cultivation volume from small to large scale can be a rather complex and challenging process when the method of aeration and mixing is different between scales. Orbitally shaken bioreactors (OSBs) utilize the same… read more here.
Keywords: perfusion; bioreactor; process; fed batch ... See more keywords
Different fermentation processes produced variants of an anti-CD52 monoclonal antibody that have divergent in vitro and in vivo characteristics
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DOI: 10.1007/s00253-017-8312-7
Abstract: The anti-CD52 antibody has already been approved for the treatment of patients with resistant chronic lymphocytic leukemia, relapsing-remitting multiple sclerosis, and has demonstrable efficacy against stem cell transplantation rejection. A CHO cell line expressing a… read more here.
Keywords: antibody; anti cd52; cd52 monoclonal; fed batch ... See more keywords