Does Serial Femtosecond Crystallography Depict State-Specific Catalytic Intermediates of the Oxygen-Evolving Complex?
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.3c00489
Abstract: Recent advances in serial femtosecond crystallography (SFX) of photosystem II (PSII), enabled by X-ray free electron lasers (XFEL), provided the first geometric models of distinct intermediates in the catalytic S-state cycle of the oxygen-evolving complex… read more here.
Keywords: oxidation; state; oxygen evolving; serial femtosecond ... See more keywords
Real-time observation of a metal complex-driven reaction intermediate using a porous protein crystal and serial femtosecond crystallography
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-024-49814-9
Abstract: Determining short-lived intermediate structures in chemical reactions is challenging. Although ultrafast spectroscopic methods can detect the formation of transient intermediates, real-space structures cannot be determined directly from such studies. Time-resolved serial femtosecond crystallography (TR-SFX) has… read more here.
Keywords: reaction; femtosecond crystallography; serial femtosecond; time ... See more keywords
Observation of early events in the photoactivation of Myxobacterial phytochrome using time-resolved serial femtosecond crystallography
Sign Up to like & getrecommendations! Published in 2025 at "Communications Chemistry"
DOI: 10.1038/s42004-025-01578-z
Abstract: Myxobacteria are non-photosynthetic, soil-dwelling bacteria distinguished by a multicellular stage in their life cycle known as fruiting bodies that are stimulated by light. Myxobacterial phytochromes are candidates for the perception of red-light. The mechanism how… read more here.
Keywords: femtosecond crystallography; resolved serial; time resolved; serial femtosecond ... See more keywords
Double-flow focused liquid injector for efficient serial femtosecond crystallography
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/srep44628
Abstract: Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge,… read more here.
Keywords: femtosecond crystallography; flow focused; serial femtosecond; double flow ... See more keywords
The Single Particles, Clusters and Biomolecules and Serial Femtosecond Crystallography instrument of the European XFEL: initial installation1
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Synchrotron Radiation"
DOI: 10.1107/s1600577519003308
Abstract: An introduction to the early operational capabilities of the Single Particles, Clusters and Biomolecules and Serial Femtosecond Crystallography (SPB/SFX) scientific instrument at the European X-ray Free Electron Laser facility is presented. read more here.
Keywords: biomolecules serial; femtosecond crystallography; single particles; serial femtosecond ... See more keywords
Experimental phase determination with selenomethionine or mercury-derivatization in serial femtosecond crystallography
Sign Up to like & getrecommendations! Published in 2017 at "IUCrJ"
DOI: 10.1107/s2052252517008557
Abstract: High-energy X-rays are essential for de novo structure determination with strong anomalous scattering from selenium or mercury. Single-wavelength anomalous diffraction phasing using selenomethionine-derivatization and mercury-soaking techniques has been successfully applied to serial femtosecond crystallography with… read more here.
Keywords: femtosecond crystallography; determination; serial femtosecond; derivatization ... See more keywords
Structural Insights into Phycocyanin Langmuir–Blodgett Multilayers via Serial Femtosecond Crystallography with X-ray Free-Electron Laser
Sign Up to like & getrecommendations! Published in 2024 at "Crystals"
DOI: 10.3390/cryst14090767
Abstract: Serial femtosecond crystallography (SFX) with X-ray free-electron lasers (XFELs) has revolutionized classical X-ray diffraction experiments by utilizing ultra-short, intense, and coherent X-ray pulses. However, the SFX approach still requires thousands of nearly identical samples, leading… read more here.
Keywords: phycocyanin langmuir; blodgett; femtosecond crystallography; langmuir blodgett ... See more keywords
Preliminary Serial Femtosecond Crystallography Studies of Myoglobin from Equine Skeletal Muscle
Sign Up to like & getrecommendations! Published in 2025 at "Crystals"
DOI: 10.3390/cryst15100905
Abstract: Myoglobin (Mb), a heme-containing protein, plays crucial roles in storing and transporting oxygen in muscle cells. Various Mb structures have been extensively determined using conventional cryogenic crystallography, providing valuable information for understanding the molecular mechanisms… read more here.
Keywords: skeletal muscle; equine skeletal; crystallography; femtosecond crystallography ... See more keywords
Sample Delivery Systems for Serial Femtosecond Crystallography at the PAL-XFEL
Sign Up to like & getrecommendations! Published in 2023 at "Photonics"
DOI: 10.3390/photonics10050557
Abstract: Serial femtosecond crystallography (SFX) using an X-ray free electron laser (XFEL) enables the determination of room-temperature structures without causing radiation damage. Using an optical pump-probe or mix-and-injection, SFX enables the intermediate state visualization of a… read more here.
Keywords: xfel; serial femtosecond; femtosecond crystallography; sample delivery ... See more keywords