High‐throughput proteomic analysis of FFPE tissue samples facilitates tumor stratification
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Oncology"
DOI: 10.1002/1878-0261.12570
Abstract: Formalin‐fixed, paraffin‐embedded (FFPE), biobanked tissue samples offer an invaluable resource for clinical and biomarker research. Here, we developed a pressure cycling technology (PCT)‐SWATH mass spectrometry workflow to analyze FFPE tissue proteomes and applied it to… read more here.
Keywords: stratification; ffpe samples; tissue samples; ffpe ... See more keywords
Extraction of RNA from formalin‐fixed, paraffin‐embedded equine placenta
Sign Up to like & getrecommendations! Published in 2019 at "Reproduction in Domestic Animals"
DOI: 10.1111/rda.13406
Abstract: Archived formalin-fixed, paraffin-embedded (FFPE) samples represent a valuable resource for the determination of gene expression for physio/pathological conditions. In the present study, we validated a protocol for the extraction of RNA from FFPE samples collected… read more here.
Keywords: fixed paraffin; ffpe samples; ffpe; paraffin embedded ... See more keywords
Abstract 1322: Development and performance of a formalin-damaged multiplexed DNA tumor mutation FFPE reference material
Sign Up to like & getrecommendations! Published in 2020 at "Cancer Research"
DOI: 10.1158/1538-7445.am2020-1322
Abstract: Tumor profiling often begins with a tissue biopsy that is formalin fixed and paraffin embedded (FFPE), a process that introduces various kinds of damage in the nucleic acids found in the tissue, however FFPE reference… read more here.
Keywords: ffpe; tumor; reference material; dna ... See more keywords
Abstract 249: A novel suite of enzyme mixes enable robust hybrid capture sequencing from low quality FFPE DNA
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Research"
DOI: 10.1158/1538-7445.am2023-249
Abstract: In cancer genomics, a common source of DNA is formalin-fixed, paraffin-embedded (FFPE) tissue from patient surgical samples, where in most cases high-quality fresh or frozen tissue samples are not available. FFPE DNA poses many notable… read more here.
Keywords: dna; quality; ffpe; mix ... See more keywords
RNA-seq from archival FFPE breast cancer samples: molecular pathway fidelity and novel discovery
Sign Up to like & getrecommendations! Published in 2019 at "BMC Medical Genomics"
DOI: 10.1186/s12920-019-0643-z
Abstract: BackgroundFormalin-fixed, paraffin-embedded (FFPE) tissues for RNA-seq have advantages over fresh frozen tissue including abundance and availability, connection to rich clinical data, and association with patient outcomes. However, FFPE-derived RNA is highly degraded and chemically modified,… read more here.
Keywords: ffpe; rna; breast cancer; gene ... See more keywords
A novel method for RNA extraction from FFPE samples reveals significant differences in biomarker expression between orthotopic and subcutaneous pancreatic cancer patient-derived xenografts
Sign Up to like & getrecommendations! Published in 2017 at "Oncotarget"
DOI: 10.18632/oncotarget.11809
Abstract: Next-generation sequencing (NGS) can identify and validate new biomarkers of cancer onset, progression and therapy resistance. Substantial archives of formalin-fixed, paraffin-embedded (FFPE) cancer samples from patients represent a rich resource for linking molecular signatures to… read more here.
Keywords: ffpe; ffpe samples; rna extraction; cancer ... See more keywords
Whole-Transcriptome Profiling on Small FFPE Samples: Which Sequencing Kit Should Be Used?
Sign Up to like & getrecommendations! Published in 2022 at "Current Issues in Molecular Biology"
DOI: 10.3390/cimb44050148
Abstract: RNA sequencing (RNA-Seq) appears as a great tool with huge clinical potential, particularly in oncology. However, sufficient sample size is often a limiting factor and the vast majority of samples from patients with cancer are… read more here.
Keywords: ffpe samples; whole transcriptome; small ffpe; ffpe ... See more keywords
Comprehensive Development and Implementation of Good Laboratory Practice for NGS Based Targeted Panel on Solid Tumor FFPE Tissues in Diagnostics
Sign Up to like & getrecommendations! Published in 2022 at "Diagnostics"
DOI: 10.3390/diagnostics12051291
Abstract: The speed, accuracy, and increasing affordability of next-generation sequencing (NGS) have revolutionized the advent of precision medicine. To date, standardized validation criteria for diagnostic accreditation do not exist due to variability across the multitude of… read more here.
Keywords: laboratory practice; ffpe; diagnostics comprehensive; comprehensive development ... See more keywords