High‐throughput proteomic analysis of FFPE tissue samples facilitates tumor stratification
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Oncology"
DOI: 10.1002/1878-0261.12570
Abstract: Formalin‐fixed, paraffin‐embedded (FFPE), biobanked tissue samples offer an invaluable resource for clinical and biomarker research. Here, we developed a pressure cycling technology (PCT)‐SWATH mass spectrometry workflow to analyze FFPE tissue proteomes and applied it to… read more here.
Keywords: stratification; ffpe samples; tissue samples; ffpe ... See more keywords
DNA Methylation in Ovarian Tumors—a Comparison Between Fresh Tissue and FFPE Samples
Sign Up to like & getrecommendations! Published in 2021 at "Reproductive Sciences"
DOI: 10.1007/s43032-021-00589-0
Abstract: Among women, ovarian cancer (OC) is one of the most severe forms of malignancy, accounting for a low 5-year survival rate, of approximately 52%. Early symptoms are unspecific and hence hard to detect. The origin… read more here.
Keywords: methylation; ffpe samples; tissue; frozen tissue ... See more keywords
Formalin-fixed paraffin-embedded (FFPE) samples help to investigate transcriptomic responses in wildlife disease.
Sign Up to like & getrecommendations! Published in 2023 at "Molecular ecology resources"
DOI: 10.1111/1755-0998.13805
Abstract: Infectious diseases impact numerous organisms. Knowledge of host-pathogen interactions and host responses to infection is crucial for conservation and management. Obtaining this knowledge quickly is made increasingly possible by a variety of genomic approaches, yet,… read more here.
Keywords: ffpe samples; embedded ffpe; formalin fixed; responses wildlife ... See more keywords
Extraction of RNA from formalin‐fixed, paraffin‐embedded equine placenta
Sign Up to like & getrecommendations! Published in 2019 at "Reproduction in Domestic Animals"
DOI: 10.1111/rda.13406
Abstract: Archived formalin-fixed, paraffin-embedded (FFPE) samples represent a valuable resource for the determination of gene expression for physio/pathological conditions. In the present study, we validated a protocol for the extraction of RNA from FFPE samples collected… read more here.
Keywords: fixed paraffin; ffpe samples; ffpe; paraffin embedded ... See more keywords
Abstract 215: A novel enzymatic library preparation workflow that dramatically reduces artifacts associated with damaged FFPE samples
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Research"
DOI: 10.1158/1538-7445.am2023-215
Abstract: Clinical oncology heavily relies on formalin-fixed, paraffin-embedded (FFPE) tissue samples for histology and molecular characterization. The chemical and physical modifications of nucleic acids introduced during fixation, storage and purification negatively impact molecular profiling and vary… read more here.
Keywords: ffpe samples; oncology; damaged ffpe; sonication ... See more keywords
Targeted RNAseq assay incorporating unique molecular identifiers for improved quantification of gene expression signatures and transcribed mutation fraction in fixed tumor samples
Sign Up to like & getrecommendations! Published in 2021 at "BMC Cancer"
DOI: 10.1186/s12885-021-07814-8
Abstract: Background Our objective was to assess whether modifications to a customized targeted RNA sequencing (RNAseq) assay to include unique molecular identifiers (UMIs) that collapse read counts to their source mRNA counts would improve quantification of… read more here.
Keywords: ffpe samples; set4 assay; rnaseq assay; unique molecular ... See more keywords
A comparative analysis of RNA sequencing methods with ribosome RNA depletion for degraded and low-input total RNA from formalin-fixed and paraffin-embedded samples.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Clinical Oncology"
DOI: 10.1200/jco.2019.37.15_suppl.e14654
Abstract: e14654 Background: Formalin-fixed and paraffin-embedded (FFPE) blocks held in clinical laboratories are an invaluable resource for clinical research, especially in the era of personalized medicine. With the development of massive parallel sequencing, RNA expression profiles… read more here.
Keywords: input; total rna; rna; depletion ... See more keywords
Formalin-Fixed Paraffin-Embedded (FFPE) samples are not a beneficial replacement for frozen tissues in fetal membrane microbiota research
Sign Up to like & getrecommendations! Published in 2022 at "PLoS ONE"
DOI: 10.1371/journal.pone.0265441
Abstract: Formalin-Fixed Paraffin-Embedded (FFPE) tissues are routinely collected, archived, and used for clinical diagnosis, including maternal and neonatal health. Applying FFPE samples to microbiota research would be beneficial to reduce preparation, storage and costs associated with… read more here.
Keywords: membrane; fetal membrane; membrane samples; ffpe samples ... See more keywords
A novel method for RNA extraction from FFPE samples reveals significant differences in biomarker expression between orthotopic and subcutaneous pancreatic cancer patient-derived xenografts
Sign Up to like & getrecommendations! Published in 2017 at "Oncotarget"
DOI: 10.18632/oncotarget.11809
Abstract: Next-generation sequencing (NGS) can identify and validate new biomarkers of cancer onset, progression and therapy resistance. Substantial archives of formalin-fixed, paraffin-embedded (FFPE) cancer samples from patients represent a rich resource for linking molecular signatures to… read more here.
Keywords: ffpe; ffpe samples; rna extraction; cancer ... See more keywords
The effect of the formalin-fixed paraffin-embedded process on salivary microbiota profiling.
Sign Up to like & getrecommendations! Published in 2023 at "Biomedical research"
DOI: 10.2220/biomedres.44.117
Abstract: In recent years, bacterial DNA in formalin-fixed paraffin-embedded (FFPE) samples has been recognized as a valuable bioresource for microbiota studies. This study aimed to examine the effect of the FFPE process on microbiota profiling to… read more here.
Keywords: ffpe samples; paraffin; microbiota; microbiota profiling ... See more keywords
Whole Transcriptome Profiling of Adrenocortical Tumors Using Formalin-Fixed Paraffin-Embedded Samples
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Endocrinology"
DOI: 10.3389/fendo.2022.808331
Abstract: Whole transcriptome profiling is a promising technique in adrenal studies; however, whole transcriptome profiling of adrenal disease using formalin-fixed paraffin-embedded (FFPE) samples has to be further explored. The aim of this study was to evaluate… read more here.
Keywords: ffpe samples; whole transcriptome; transcriptome profiling; adrenocortical tumors ... See more keywords