High‐throughput proteomic analysis of FFPE tissue samples facilitates tumor stratification
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Oncology"
DOI: 10.1002/1878-0261.12570
Abstract: Formalin‐fixed, paraffin‐embedded (FFPE), biobanked tissue samples offer an invaluable resource for clinical and biomarker research. Here, we developed a pressure cycling technology (PCT)‐SWATH mass spectrometry workflow to analyze FFPE tissue proteomes and applied it to… read more here.
Keywords: stratification; ffpe samples; tissue samples; ffpe ... See more keywords
On-tissue amidation of sialic acid with aniline for sensitive imaging of sialylated N-glycans from FFPE tissue sections via MALDI mass spectrometry
Sign Up to like & getrecommendations! Published in 2022 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-022-03894-y
Abstract: Spatial visualization of glycans within clinical tissue samples is critical for discovery of disease-relevant glycan dysregulations. Herein, we develop an on-tissue derivatization strategy for sensitive spatial visualization of N-glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections,… read more here.
Keywords: tissue sections; tissue; sialic acid; amidation ... See more keywords
Comparison of different digestion methods for proteomic analysis of isolated cells and FFPE tissue samples.
Sign Up to like & getrecommendations! Published in 2021 at "Talanta"
DOI: 10.1016/j.talanta.2021.122568
Abstract: Proteomics of human tissues and isolated cellular subpopulations create new opportunities for therapy and monitoring of a patients' treatment in the clinic. Important considerations in such analysis include recovery of adequate amounts of protein for… read more here.
Keywords: tissue samples; analysis; digestion; method ... See more keywords
Setting a diagnostic benchmark for tumor BRCA testing: Detection of BRCA1 and BRCA2 large genomic rearrangements in FFPE tissue - A pilot study.
Sign Up to like & getrecommendations! Published in 2021 at "Experimental and molecular pathology"
DOI: 10.1016/j.yexmp.2021.104705
Abstract: PARP inhibitors are used for treatment of tumors lacking function of the double-strand DNA break repair proteins BRCA1 or BRCA2 and are already approved for several cancer types. Thus, it is clinically crucial to determine… read more here.
Keywords: large genomic; brca1; ffpe tissue; brca1 brca2 ... See more keywords
A High-Throughput Workflow for FFPE Tissue Proteomics.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.3c00099
Abstract: Laser capture microdissection (LCM) has become an indispensable tool for mass spectrometry-based proteomic analysis of specific regions obtained from formalin-fixed paraffin-embedded (FFPE) tissue samples in both clinical and research settings. Low protein yields from LCM… read more here.
Keywords: ffpe tissue; sample processing; trypsin digestion; high throughput ... See more keywords
Small Nucleolar RNA Score: An Assay to Detect Formalin-Overfixed Tissue
Sign Up to like & getrecommendations! Published in 2018 at "Biopreservation and Biobanking"
DOI: 10.1089/bio.2018.0042
Abstract: Although there are millions of formalin-fixed paraffin-embedded (FFPE) tissue blocks potentially available for scientific research, many are of questionable quality, partly due to unknown fixation conditions. We analyzed FFPE tissue biospecimens as part of the… read more here.
Keywords: formalin; rna; tissue; ffpe tissue ... See more keywords
Abstract 241: Evaluation of single cell RNA sequencing from FFPE tissue using 10x Genomics Chromium Fixed RNA Profiling
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Research"
DOI: 10.1158/1538-7445.am2023-241
Abstract: Single cell RNA sequencing (scRNA-seq) uncovers tissue heterogeneity by providing gene expression measurements in individual cells within the tissue. This level of resolution allows for a clearer understanding of specific cellular functions, tissue complexity, and… read more here.
Keywords: cell; ffpe tissue; single cell; fixed rna ... See more keywords
Potential of STAT Somatic Mutation Testing at Resection.
Sign Up to like & getrecommendations! Published in 2018 at "Clinical chemistry"
DOI: 10.1373/clinchem.2017.285759
Abstract: To the Editor: Molecular testing is used for the management of various cancers as more targeted therapies are becoming available as treatment options. The complexity of treatment strategies for cancer patients has made accurate and… read more here.
Keywords: mutation; somatic mutation; mutation testing; ffpe tissue ... See more keywords