Fusion transcript discovery using RNA sequencing in formalin-fixed paraffin-embedded specimen.
Sign Up to like & getrecommendations! Published in 2021 at "Critical reviews in oncology/hematology"
DOI: 10.1016/j.critrevonc.2021.103303
Abstract: Chimeric transcripts are critical for diagnosis or prognosis and could constitute effective therapeutic targets. Fresh tissues are the major source for the identification of these fusion transcripts. The quality and quantity of the extracted RNA… read more here.
Keywords: fusion transcript; transcript discovery; ffpe tissues; formalin fixed ... See more keywords
Highly Accurate and Robust Absolute Quantification of Target Proteins in Formalin-Fixed Paraffin-Embedded (FFPE) Tissues by LC-MS.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c03473
Abstract: Accurate, absolute liquid chromatography-mass spectrometry (LC-MS)-based quantification of target proteins in formalin-fixed paraffin-embedded (FFPE) tissues would greatly expand sample availability for pharmaceutical/clinical investigations but remains challenging owing to the following issues: (i) efficient/quantitative recovery of… read more here.
Keywords: quantification; ffpe tissues; target proteins; quantification target ... See more keywords
In-Depth Mass Spectrometry-Based Proteomics of Formalin-Fixed, Paraffin-Embedded Tissues with a Spatial Resolution of 50-200 μm.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.2c00409
Abstract: Formalin-fixed, paraffin-embedded (FFPE) tissues are banked in large repositories to cost-effectively preserve valuable specimens for later study. With the rapid growth of spatial proteomics, FFPE tissues can serve as a more accessible alternative to more… read more here.
Keywords: formalin fixed; ffpe tissues; resolution; paraffin embedded ... See more keywords
54 Patient stratification using clinical proteomics – validated multiplexed MRM assays to quantify HER2 and other biomarkers in clinical FFPE tissues
Sign Up to like & getrecommendations! Published in 2020 at "Journal for ImmunoTherapy of Cancer"
DOI: 10.1136/jitc-2020-sitc2020.0054
Abstract: Background The advent of precision oncology has led a shift towards biomarker-driven clinical trial designs and molecular profiling of individual patients. Identification of patients with the target biomarker profile may be useful in guiding patient… read more here.
Keywords: mrm assays; mrm; multiplexed mrm; biomarker ... See more keywords
Quantitative Analysis of Tyrosine Phosphorylation from FFPE Tissues Reveals Patient-Specific Signaling Networks
Sign Up to like & getrecommendations! Published in 2021 at "Cancer Research"
DOI: 10.1158/0008-5472.can-21-0214
Abstract: This study reports a highly sensitive method utilizing FFPE tissues to identify dysregulated signaling networks in patient tumors, opening the door for direct translational insights from FFPE tumor tissue banks in hospitals. Human tissue samples… read more here.
Keywords: ffpe tissues; tyrosine phosphorylation; signaling networks; tissue ... See more keywords
Epigenomic profiling of archived FFPE tissues by enhanced PAT-ChIP (EPAT-ChIP) technology
Sign Up to like & getrecommendations! Published in 2018 at "Clinical Epigenetics"
DOI: 10.1186/s13148-018-0576-y
Abstract: BackgroundThe introduction of pathology tissue-chromatin immunoprecipitation (PAT-ChIP), a technique allowing chromatin immunoprecipitation (ChIP) from formalin-fixed paraffin-embedded (FFPE) tissues, has extended the application of chromatin studies to clinical patient samples. However, extensive crosslinking introduced during routine… read more here.
Keywords: epat chip; chromatin; ffpe tissues; pathology ... See more keywords
Biomarker Analysis of Formalin-Fixed Paraffin-Embedded Clinical Tissues Using Proteomics
Sign Up to like & getrecommendations! Published in 2023 at "Biomolecules"
DOI: 10.3390/biom13010096
Abstract: The relatively recent developments in mass spectrometry (MS) have provided novel opportunities for this technology to impact modern medicine. One of those opportunities is in biomarker discovery and diagnostics. Key developments in sample preparation have… read more here.
Keywords: formalin fixed; biomarker analysis; ffpe tissues; paraffin embedded ... See more keywords
The Current State of Chromatin Immunoprecipitation (ChIP) from FFPE Tissues
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23031103
Abstract: Cancer cells accumulate epigenomic aberrations that contribute to cancer initiation and progression by altering both the genomic stability and the expression of genes. The awareness of such alterations could improve our understanding of cancer dynamics… read more here.
Keywords: chromatin; immunoprecipitation chip; chromatin immunoprecipitation; ffpe tissues ... See more keywords
Z Probe, An Efficient Tool for Characterizing Long Non-Coding RNA in FFPE Tissues
Sign Up to like & getrecommendations! Published in 2018 at "Non-Coding RNA"
DOI: 10.3390/ncrna4030020
Abstract: Formalin-fixed paraffin embedded (FFPE) tissues are a valuable resource for biomarker discovery in order to understand the etiology of different cancers and many other diseases. Proteins are the biomarkers of interest with respect to FFPE… read more here.
Keywords: ffpe tissues; long non; non coding; coding rna ... See more keywords