Metabolic flux of N10-formyltetrahydrofolate plays a critical role in the fidelity of translation initiation in Escherichia coli.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of molecular biology"
DOI: 10.1016/j.jmb.2020.08.003
Abstract: One-carbon metabolism produces methionine and N10-formyl-tetrahydrofolate (N10-fTHF) required for aminoacylation and formylation of initiator tRNA (i-tRNA), respectively. In Escherichia coli, N10-fTHF is made from 5, 10-methylene-THF by a two-step reaction using 5,10-methylene-THF dehydrogenase/cyclohydrolase (FolD). The… read more here.
Keywords: initiation; escherichia coli; fidelity translation; trna ... See more keywords
Assessing the fidelity of translation of non‐clinical assays: a Pharma perspective
Sign Up to like & getrecommendations! Published in 2022 at "British Journal of Pharmacology"
DOI: 10.1111/bph.15796
Abstract: Advances in non‐clinical in vitro models, higher throughput approaches and the promise of human‐derived preparations require methods to reliably assess the fidelity of translation of such assays, compared with in vivo models and clinical studies.… read more here.
Keywords: assessing fidelity; non clinical; clinical assays; fidelity translation ... See more keywords