Karyotyping of Fusarium oxysporum by Pulsed-Field Gel Electrophoresis and the Germ Tube Burst Method.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1795-3_3
Abstract: In fungi, karyotyping is fundamental to understanding their genome organization. It is also essential to study various genome- or chromosome-related topics such as karyotype polymorphisms and supernumerary or pathogenicity chromosomes. Here, we describe the protocols… read more here.
Keywords: gel electrophoresis; germ tube; pulsed field; tube burst ... See more keywords
Analyzing Copy Number Variation Using Pulsed-Field Gel Electrophoresis: Providing a Genetic Diagnosis for FSHD1.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6442-0_7
Abstract: The myopathy facioscapulohumeral muscular dystrophy type 1 (FSHD1) is caused by copy number variation of the D4Z4 macrosatellite repeat on chromosome 4. In unaffected individuals the number of 3.3 kb D4Z4 units varies between 8… read more here.
Keywords: copy number; number variation; gel electrophoresis; pulsed field ... See more keywords
Using Pulsed-Field Gel Electrophoresis to Analyze Schizosaccharomyces pombe Chromosomes and Chromosomal Elements.
Sign Up to like & getrecommendations! Published in 2018 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot092023
Abstract: Pulsed field gel electrophoresis (PFGE) uses alternatively oriented pulsed electrical fields to separate large DNA molecules. Here, we describe PFGE protocols and conditions for separating and visualizing chromosomes between 0.5 and 6 Mb (optimal for… read more here.
Keywords: chromosomal elements; pulsed field; field gel; gel electrophoresis ... See more keywords
Genotyping and phenotyping of Lactococcus garvieae isolates from fish by pulse-field gel electrophoresis (PFGE) and electron microscopy indicate geographical and capsular variations.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of fish diseases"
DOI: 10.1111/jfd.13601
Abstract: Lactococcus garvieae is the etiological agent of Lactococcosis, an evolving disease affecting many fish species and causing significant economic losses worldwide. Assessing pathogen relatedness and bacterial population structure is critical for determining the epidemiology of… read more here.
Keywords: lactococcus garvieae; field gel; garvieae isolates; microscopy ... See more keywords
Tracing of Listeria monocytogenes Contamination Routes in Fermented Sausage Production Chain by Pulsed-Field Gel Electrophoresis Typing
Sign Up to like & getrecommendations! Published in 2018 at "Foods"
DOI: 10.3390/foods7120198
Abstract: In this study, the presence of Listeria monocytogenes was assessed along the production process of fermented sausages in a small-scale facility. Following the isolation of the pathogen from the final product (ISO 11290-1), retrospective sampling… read more here.
Keywords: sausage production; production; gel electrophoresis; pulsed field ... See more keywords