A fast and efficient stabilization of firefly luciferase on MIL-53(Al) via surface adsorption mechanism
Sign Up to like & getrecommendations! Published in 2019 at "Research on Chemical Intermediates"
DOI: 10.1007/s11164-019-03748-w
Abstract: Stabilization and immobilization on a proper support is essential in processing and production of enzymes. Luciferase is an unstable, thermally sensitive, and light-emitting enzyme that requires stabilization for efficiency and productivity in bio-luminescence reactions. In… read more here.
Keywords: stabilization firefly; firefly luciferase; mil;
Histidine substitution in the most flexible fragments of firefly luciferase modifies its thermal stability.
Sign Up to like & getrecommendations! Published in 2017 at "Archives of biochemistry and biophysics"
DOI: 10.1016/j.abb.2017.07.003
Abstract: Molecular dynamics (MD) at two temperatures of 300 and 340 K identified two histidine residues, His461 and His489, in the most flexible regions of firefly luciferase, a light emitting enzyme. We therefore designed four protein mutants… read more here.
Keywords: temperature; firefly luciferase; histidine substitution; substitution ... See more keywords
Bifunctional role of leucine 300 of firefly luciferase in structural rigidity.
Sign Up to like & getrecommendations! Published in 2017 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2017.03.069
Abstract: Firefly luciferase is susceptible to thermal inactivation, thereby its intracellular half-life decreased. Previous reports indicated that L300R mutation (LRR mutant) in E354R/Arg356 double mutant (ERR mutant) from Lampyris turkestanicus luciferase has increased its thermal stability… read more here.
Keywords: leucine 300; rigidity; firefly luciferase;
Identification of 2-Benzylidene-tetralone Derivatives as Highly Potent and Reversible Firefly Luciferase Inhibitors.
Sign Up to like & getrecommendations! Published in 2022 at "ACS medicinal chemistry letters"
DOI: 10.1021/acsmedchemlett.1c00671
Abstract: The extensive applications of Firefly luciferase (Fluc) in numerous biological, biomedical, and clinical investigations rendered an urgent need for efficient and biocompatible Fluc inhibitors for the construction of novel assay platforms. Herein we describe the… read more here.
Keywords: firefly luciferase; tetralone derivatives; benzylidene tetralone; derivatives highly ... See more keywords
A Firefly Luciferase Dual Color Bioluminescence Reporter Assay Using Two Substrates To Simultaneously Monitor Two Gene Expression Events
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-24278-2
Abstract: Effective methods for monitoring eukaryotic gene expression and regulation based on bioluminescence - the emission of light by living organisms - are well established. Typically, the expression of a gene of interest is reported on… read more here.
Keywords: reporter; firefly luciferase; gene expression; gene ... See more keywords
Hepa1-6-FLuc cell line with the stable expression of firefly luciferase retains its primary properties with promising bioluminescence imaging ability
Sign Up to like & getrecommendations! Published in 2018 at "Oncology Letters"
DOI: 10.3892/ol.2018.8132
Abstract: Reliable animal models are required for the in vivo study of the molecular mechanisms and effects of chemotherapeutic drugs in hepatocarcinoma. In vivo tracing techniques based on firefly luciferase (FLuc) may optimize the non-invasive monitoring… read more here.
Keywords: firefly luciferase; expression; cell; hepa1 fluc ... See more keywords