The proline‐rich domain of fission yeast WASp (Wsp1p) interacts with actin filaments and inhibits actin polymerization
Sign Up to like & getrecommendations! Published in 2023 at "FEBS Letters"
DOI: 10.1002/1873-3468.14571
Abstract: Members of the Wiskott–Aldrich Syndrome protein (WASp) family activate Arp2/3 complex (actin‐related proteins 2 and 3 complex) to form actin filament branches. The proline‐rich domain (PRD) of WASp contributes to branching nucleation, and the PRD… read more here.
Keywords: actin filaments; rich domain; proline rich; actin ... See more keywords
Absence of the Rpb9 subunit of RNA polymerase II reduces the chronological life span in fission yeast
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Basic Microbiology"
DOI: 10.1002/jobm.202200036
Abstract: Fission yeast RNA polymerase II consists of 12 subunits, Rpb1‐Rpb12. Among these subunits, Rpb9 is the only subunit whose absence does not cause lethality under optimum growth conditions in fission yeast. However, an rpb9 null… read more here.
Keywords: chronological life; fission; rpb9 subunit; fission yeast ... See more keywords
Sporulation: A response to starvation in the fission yeast Schizosaccharomyces pombe
Sign Up to like & getrecommendations! Published in 2022 at "MicrobiologyOpen"
DOI: 10.1002/mbo3.1303
Abstract: The fission yeast Schizosaccharomyces pombe employs two main strategies to adapt to the environment and survive when starved for nutrients. The strategies employ sporulation via sexual differentiation and extension of the chronological lifespan. When a… read more here.
Keywords: yeast schizosaccharomyces; sporulation; starvation; fission yeast ... See more keywords
Schizosaccharomyces lindneri sp. nov., a fission yeast occurring in honey.
Sign Up to like & getrecommendations! Published in 2023 at "Yeast"
DOI: 10.1002/yea.3857
Abstract: Two strains of fission yeast were isolated from honey. They differ from the type strain of Schizosaccharomyces octosporus by three substitutions in the D1/D2 domain of the nuclear 26S large subunit ribosomal RNA (rRNA) gene sequence,… read more here.
Keywords: schizosaccharomyces lindneri; honey; lindneri nov; fission yeast ... See more keywords
Methods to Assess Phosphodiesterase and/or Adenylyl Cyclase Activity Via Heterologous Expression in Fission Yeast.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2245-2_6
Abstract: Heterologous expression of cyclic nucleotide phosphodiesterases (PDEs) and adenylyl cyclases (ACs) in the fission yeast Schizosaccharomyces pombe can be used in combination with PKA-repressed reporters to either carry out high throughput screens for small molecule… read more here.
Keywords: heterologous expression; fission yeast; activity;
Chromatin Immunoprecipitation of Histone Modifications in Fission Yeast.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6630-1_12
Abstract: Chromatin immunoprecipitation (ChIP) is a sensitive, accurate, and reliable technique widely used to analyze protein-DNA interactions at specific binding sites in vivo. It has been a particularly powerful technique for mapping of histone modification patterns… read more here.
Keywords: fission yeast; histone modifications; histone; chromatin immunoprecipitation ... See more keywords
Efficient Depletion of Fission Yeast Condensin by Combined Transcriptional Repression and Auxin-Induced Degradation.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9520-2_3
Abstract: Structural maintenance of chromosomes (SMC) complexes play pivotal roles in controlling chromatin organization. Condensin is an essential SMC complex that compacts chromatin to form condensed chromosomes in mitosis. Complete condensin inactivation is necessary to reveal… read more here.
Keywords: repression auxin; condensin; depletion; fission yeast ... See more keywords
Negative control of cytokinesis by stress-activated MAPK signaling.
Sign Up to like & getrecommendations! Published in 2021 at "Current genetics"
DOI: 10.1007/s00294-021-01155-6
Abstract: Mitogen-activated protein kinase (MAPK) signalling pathways regulate multiple cellular functions in eukaryotic organisms in response to environmental cues, including the dynamic remodeling of the actin cytoskeleton. The fission yeast S. pombe is an optimal model… read more here.
Keywords: stress activated; cytokinesis; car; negative control ... See more keywords
Temperature sensitive point mutations in fission yeast tropomyosin have long range effects on the stability and function of the actin-tropomyosin copolymer
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and Biophysical Research Communications"
DOI: 10.1016/j.bbrc.2017.10.109
Abstract: The actin cytoskeleton is modulated by regulatory actin-binding proteins which fine-tune the dynamic properties of the actin polymer to regulate function. One such actin-binding protein is tropomyosin (Tpm), a highly-conserved alpha-helical dimer which stabilises actin… read more here.
Keywords: point mutations; tropomyosin; temperature sensitive; function ... See more keywords
Modeling the Dynamics of Cdc42 Oscillation in Fission Yeast.
Sign Up to like & getrecommendations! Published in 2018 at "Biophysical journal"
DOI: 10.1016/j.bpj.2017.12.007
Abstract: Regulation of polarized cell growth is essential for many cellular processes, including spatial coordination of cell morphology changes during growth and division. We present a mathematical model of the core mechanism responsible for the regulation… read more here.
Keywords: cell; gef; model; growth ... See more keywords
Formin Cdc12's specific actin assembly properties are tailored for cytokinesis in fission yeast.
Sign Up to like & getrecommendations! Published in 2021 at "Biophysical journal"
DOI: 10.1016/j.bpj.2021.06.023
Abstract: Formins generate unbranched actin filaments by a conserved, processive actin assembly mechanism. Most organisms express multiple formin isoforms that mediate distinct cellular processes and facilitate actin filament polymerization by significantly different rates, but how these… read more here.
Keywords: actin; actin assembly; fission yeast; cytokinesis ... See more keywords