Expanding the Dimensions of Single-Cell Multi-Omics To Include Mitochondrial DNA through Fixation and Permeabilization.
Sign Up to like & getrecommendations! Published in 2025 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.4c06777
Abstract: Single-cell multi-omics has transformed our understanding of cellular heterogeneity, yet incorporating mitochondrial DNA (mtDNA) remains challenging. Recent studies underscore the critical role of fixation and permeabilization techniques in preserving sample integrity, optimizing Tn5 tagmentation, and… read more here.
Keywords: multi omics; single cell; fixation permeabilization; cell ... See more keywords
Influence of the fixation/permeabilization step on peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) for the detection of bacteria
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DOI: 10.1371/journal.pone.0196522
Abstract: Fluorescence in situ Hybridization (FISH) is a versatile, widespread and widely- used technique in microbiology. The first step of FISH—fixation/permeabilization—is crucial to the outcome of the method. This work aimed to systematically evaluate fixation/permeabilization protocols… read more here.
Keywords: fixation permeabilization; fluorescence situ; situ hybridization; permeabilization ... See more keywords
Influence of Fixation and Permeabilization on the Mass Density of Single Cells: A Surface Plasmon Resonance Imaging Study
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DOI: 10.3389/fchem.2019.00588
Abstract: Fixation and permeabilization of cells and tissues are essential processes in biological techniques like immunofluorescence and immunohistochemistry for cell biology studies. In typical procedures, the biological samples are treated by paraformaldehyde and Triton X-100 to… read more here.
Keywords: fixation permeabilization; mass density; permeabilization; fixation ... See more keywords